Chemical Synthesis of K33 Acetylated SUMO Protein

doi:10.7503/cjcu20160417

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院强磁场科学中心

	Chemical Synthesis of K33 Acetylated SUMO Protein
	Wang Yehai 1; Kong Yifu 1; Chen Chenchen 2; Li Yiming 1
刊名	CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
	2016-11-10
卷号	37 期号:11 页码:1987-1992
关键词	Small Ubquitin-related Modifier Acetylation High Temperature Assisted Solid-phase Peptide Syn-thesis Peptide Hydrazide Native Chemical Ligation
DOI	10.7503/cjcu20160417
文献子类	Article
英文摘要	Sumoylated protein was found to reduce the protein binding force with the SUMO. interaction motif (SIM) of effector protein when K33 of SUMO was acetylated. To further study the structures and mechanism, a large amount of uniformity acetylated SUMO protein was greatly needed. In this work, SUMO protein and K33 acetylated SUMO protein was efficiently obtained using high temperature assisted solid. phase peptide synthesis (SPPS) technology combined with peptide hydrazide ligation. SUMO and K33 acetylated SUMO was identified with corrected molecular weight by the SDS-PAGE. It was also confirmed that the chemically synthetic protein and biologically expressed SUMO2 own similar homogeneity and secondary structure by the CD analysis. These results provided a foundation for the follow. up study of K33 acetylated SUMO protein.
WOS关键词	PEPTIDE HYDRAZIDES ; INTERACTION NETWORKS ; DNA-REPAIR ; UBIQUITIN ; SUMOYLATION ; LIGATION ; CONJUGATION ; PHOSPHORYLATION ; MECHANISMS ; INSIGHTS
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000388831400008
资助机构	National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; 21572043) ; 21572043) ; 21572043) ; 21572043) ; 21572043) ; 21572043) ; 21572043) ; 21572043) ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; National Natural Science Foundation of China(21372058 ; 21572043) ; 21572043) ; 21572043) ; 21572043) ; 21572043) ; 21572043) ; 21572043) ; 21572043)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/30247]
专题	合肥物质科学研究院_中科院强磁场科学中心
作者单位	1.Hefei Univ Technol, Sch Biol & Med Engn, Hefei 230009, Peoples R China 2.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Wang Yehai,Kong Yifu,Chen Chenchen,et al. Chemical Synthesis of K33 Acetylated SUMO Protein[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2016,37(11):1987-1992.
APA	Wang Yehai,Kong Yifu,Chen Chenchen,&Li Yiming.(2016).Chemical Synthesis of K33 Acetylated SUMO Protein.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,37(11),1987-1992.
MLA	Wang Yehai,et al."Chemical Synthesis of K33 Acetylated SUMO Protein".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 37.11(2016):1987-1992.