Sub-molecular features of single proteins in solution resolved with scanning tunneling microscopy

doi:10.1007/s12274-016-1141-7

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院强磁场科学中心

	Sub-molecular features of single proteins in solution resolved with scanning tunneling microscopy
	Wang, Jihao 1,2,3; Zhang, Lei 1,2; Hu, Chen 1,2; Liu, Qingsong 1,2; Hou, Yubin 1,2; Zhang, Xin 1,2; Lu, Qingyou 1,2,3,4
刊名	NANO RESEARCH
	2016-09-01
卷号	9 期号:9 页码:2551-2560
关键词	Liquid-phase Scanning Tunneling Microscopy (Stm) Stm Imaging Under Physiological Conditions Protein Molecule Sub-molecular Resolution
DOI	10.1007/s12274-016-1141-7
文献子类	Article
英文摘要	Scanning tunneling microscopy (STM) can be used to image individual biological molecules, such as proteins, in vacuum or air. This requires sample dehydration and thus may not reflect the native state of the molecule. Extensive efforts have been made to image single proteins in solution using STM; however, the images have revealed only round or oval shapes with no sub-molecular details. Here, we present the sub-molecular features of streptavidin proteins under physiological conditions using a homebuilt low-leakage-current and highstability liquid phase STM. The N-lobe, C-lobe, and C-terminal tail of the epidermal growth factor receptor kinase domains were also resolved in solution. Our results demonstrate that the structure, morphology, and dynamics of a protein molecule can be examined under physiological conditions by the STM.
WOS关键词	ATOMIC-FORCE MICROSCOPY ; ANTIBODY-ANTIGEN BINDING ; IN-SITU STM ; SUBMOLECULAR RESOLUTION ; ELECTRODE SURFACES ; DNA-MOLECULES ; STREPTAVIDIN ; HYDRATION ; ADSORPTION ; MONOLAYERS
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
WOS记录号	WOS:000382882200003
资助机构	Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; U1232210 ; U1232210 ; U1232210 ; U1232210 ; U1232210 ; U1232210 ; U1232210 ; U1232210 ; 11204306 ; 11204306 ; 11204306 ; 11204306 ; 11204306 ; 11204306 ; 11204306 ; 11204306 ; 11374278) ; 11374278) ; 11374278) ; 11374278) ; 11374278) ; 11374278) ; 11374278) ; 11374278) ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; Chinese Academy of Sciences "Hundred Talent program" ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; National Natural Science Foundation of China(U1532151 ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Chinese national high magnetic field facilities ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; Fundamental Research Funds for the Central Universities(WK2340000035) ; U1232210 ; U1232210 ; U1232210 ; U1232210 ; U1232210 ; U1232210 ; U1232210 ; U1232210 ; 11204306 ; 11204306 ; 11204306 ; 11204306 ; 11204306 ; 11204306 ; 11204306 ; 11204306 ; 11374278) ; 11374278) ; 11374278) ; 11374278) ; 11374278) ; 11374278) ; 11374278) ; 11374278)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/24084]
专题	合肥物质科学研究院_中科院强磁场科学中心
作者单位	1.Chinese Acad Sci, High Field Magnet Lab, Hefei 230026, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Peoples R China 3.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Peoples R China 4.Nanjing Univ, Collaborat Innovat Ctr Adv Microstruct, Nanjing 210093, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jihao,Zhang, Lei,Hu, Chen,et al. Sub-molecular features of single proteins in solution resolved with scanning tunneling microscopy[J]. NANO RESEARCH,2016,9(9):2551-2560.
APA	Wang, Jihao.,Zhang, Lei.,Hu, Chen.,Liu, Qingsong.,Hou, Yubin.,...&Lu, Qingyou.(2016).Sub-molecular features of single proteins in solution resolved with scanning tunneling microscopy.NANO RESEARCH,9(9),2551-2560.
MLA	Wang, Jihao,et al."Sub-molecular features of single proteins in solution resolved with scanning tunneling microscopy".NANO RESEARCH 9.9(2016):2551-2560.