Casp3/7-Instructed Intracellular Aggregation of Fe3O4 Nanoparticles Enhances T-2 MR Imaging of Tumor Apoptosis

doi:10.1021/acs.nanolett.6b00331

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院强磁场科学中心

	Casp3/7-Instructed Intracellular Aggregation of Fe3O4 Nanoparticles Enhances T-2 MR Imaging of Tumor Apoptosis
	Yuan, Yue 1; Ding, Zhanling 1; Qian, Junchao 2; Zhang, Jia 1; Xu, Jinyong 2; Dong, Xuejiao 1; Han, Tao 1; Ge, Shuchao 3; Luo, Yufeng 1; Wang, Yuwei 1
刊名	NANO LETTERS
	2016-04-01
卷号	16 期号:4 页码:2686-2691
关键词	Caspase 3/7 Aggregation Ultrasmall Superparamagnetic Iron Oxide Transverse Relaxation Magnetic Resonance Imaging
DOI	10.1021/acs.nanolett.6b00331
文献子类	Article
英文摘要	Large magnetic nanoparticles or aggregates are advantageous in their magnetic resonance properties over ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles (NPs), but the former are cleared faster from the blood pool. Therefore, the "smart" strategy of intracellular aggregation of USPIO NPs is required for enhanced T-2-weighted MR imaging. Herein, employing an enzyme-instructed condensation reaction, we rationally designed a small molecule Ac-Asp-Glu-Val-Asp-Cys(StBu)-Lys-CBT (1) to covalently modify USPIO NPs to prepare monodispersive Fe3O4@1 NPs. In vitro results showed that Fe3O4@1 NPs could be subjected to caspase 3 (Casp3)-instructed aggregation. T-2 phantom MR imaging showed that the transverse molar relaxivity (r(2)) of Fe3O4@1 NPs with Casp3 or apoptotic HepG2 cells was significantly larger than those of control groups. In vivo tumor MR imaging results indicated that Fe3O4@1 NPs could be specifically applied for enhanced T-2 MR imaging of tumor apoptosis. We propose that the enzyme-instructed intracellular aggregation of Fe3O4 NPs could be a novel strategy for the design of "smart" probes for efficient T-2 MR imaging of in vivo biomarkers.
WOS关键词	IRON-OXIDE NANOPARTICLES ; DETECT PROTEASE ACTIVITY ; IN-VIVO ; CONTRAST AGENT ; HYPERPOLARIZED XENON ; F-19 NANOPARTICLES ; CANCER ; CELLS ; FERUMOXTRAN-10 ; CONDENSATION
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
WOS记录号	WOS:000374274600083
资助机构	Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; 21375121 ; 21375121 ; 21375121 ; 21375121 ; 21375121 ; 21375121 ; 21375121 ; 21375121 ; U1232212 ; U1232212 ; U1232212 ; U1232212 ; U1232212 ; U1232212 ; U1232212 ; U1232212 ; U1332142) ; U1332142) ; U1332142) ; U1332142) ; U1332142) ; U1332142) ; U1332142) ; U1332142) ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Collaborative Innovation Center of Suzhou Nano Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Major Program of Development Foundation of Hefei Center for Physical Science and Technology ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Hefei Science Center CAS(2015HSC-UP012) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; Fundamental Research Funds for the Central Universities(WK2060190054) ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; National Natural Science Foundation of China(U1532144 ; 21375121 ; 21375121 ; 21375121 ; 21375121 ; 21375121 ; 21375121 ; 21375121 ; 21375121 ; U1232212 ; U1232212 ; U1232212 ; U1232212 ; U1232212 ; U1232212 ; U1232212 ; U1232212 ; U1332142) ; U1332142) ; U1332142) ; U1332142) ; U1332142) ; U1332142) ; U1332142) ; U1332142)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/22370]
专题	合肥物质科学研究院_中科院强磁场科学中心
作者单位	1.Univ Sci & Technol China, Dept Chem, CAS Key Lab Soft Matter Chem, 96 Jinzhai Rd, Hefei 230026, Anhui, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, High Field Magnet Lab, 350 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Yue,Ding, Zhanling,Qian, Junchao,et al. Casp3/7-Instructed Intracellular Aggregation of Fe3O4 Nanoparticles Enhances T-2 MR Imaging of Tumor Apoptosis[J]. NANO LETTERS,2016,16(4):2686-2691.
APA	Yuan, Yue.,Ding, Zhanling.,Qian, Junchao.,Zhang, Jia.,Xu, Jinyong.,...&Liang, Gaolin.(2016).Casp3/7-Instructed Intracellular Aggregation of Fe3O4 Nanoparticles Enhances T-2 MR Imaging of Tumor Apoptosis.NANO LETTERS,16(4),2686-2691.
MLA	Yuan, Yue,et al."Casp3/7-Instructed Intracellular Aggregation of Fe3O4 Nanoparticles Enhances T-2 MR Imaging of Tumor Apoptosis".NANO LETTERS 16.4(2016):2686-2691.