Conversion of Corn Stalk to Ethanol by One-Step Process using an Alcohol Dehydrogenase Mutant of Phanerochaete chrysosporium

doi:10.15376/biores.11.4.9940-9955

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院等离子体物理研究所

	Conversion of Corn Stalk to Ethanol by One-Step Process using an Alcohol Dehydrogenase Mutant of Phanerochaete chrysosporium
	Liu, Lei 1; Li, Ping 1; Qin, Ge 1; Yan, Yue 1; Li, Yi 1; Yao, JianMing 2; Wang, Hailei 1,3
刊名	BIORESOURCES
	2016-11-01
卷号	11 期号:4 页码:9940-9955
关键词	Ethanol Phanerochaete Chrysosporium Alcohol Dehydrogenase Mn-dependent Peroxidase Cellulase
DOI	10.15376/biores.11.4.9940-9955
文献子类	Article
英文摘要	The potential of Phanerochaete chrysosporium in ethanol fermentation was evaluated. During the initial submerged cultivation, 1.76 g/L ethanol was obtained using glucose as substrate. After mutation, the ethanol concentration of an alcohol dehydrogenase (ADH) mutant reached 5.02 g/L. Both base transition and nine-base frame shift mutation occurred in the ADH gene of the mutant, changing the secondary and tertiary structures of ADH, as well as increasing the ADH activity during cultivation. Moreover, P. chrysosporium converted corn stalk to ethanol by a one-step process. After statistical optimizations, 0.26 g/g center dot substrate of ethanol yield was obtained on day 10. During the fermentation, the maximum lignin peroxidase, Mn-dependent peroxidase, and cellulase activities were 29.0 U/L, 256.5 U/L, and 40 U/mL, respectively, thus explaining why the fungus directly ferments corn stalk to ethanol. This study is the first report of the conversion of corn stalk without pretreatment to ethanol using a white-rot fungus.
WOS关键词	WHEAT-STRAW ; SACCHAROMYCES-CEREVISIAE ; CATABOLITE REPRESSION ; FUEL ETHANOL ; FERMENTATION ; LIGNIN ; PRETREATMENT ; FEEDSTOCKS ; DISRUPTION ; CELLULASE
WOS研究方向	Materials Science
语种	英语
WOS记录号	WOS:000391801300135
资助机构	National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; National Science Foundation of China(51008119) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; PhD Research Startup Foundation(5101049170143) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17) ; Young Scholar's Foundation of Henan Normal University(2015QK17)
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/21707]
专题	合肥物质科学研究院_中科院等离子体物理研究所
作者单位	1.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China 2.Chinese Acad Sci, Inst Plasma Phys, Hefei 230031, Peoples R China 3.Univ Henan Prov, Key Lab Microorganisms & Funct Mol, Xinxiang 453007, Peoples R China
推荐引用方式 GB/T 7714	Liu, Lei,Li, Ping,Qin, Ge,et al. Conversion of Corn Stalk to Ethanol by One-Step Process using an Alcohol Dehydrogenase Mutant of Phanerochaete chrysosporium[J]. BIORESOURCES,2016,11(4):9940-9955.
APA	Liu, Lei.,Li, Ping.,Qin, Ge.,Yan, Yue.,Li, Yi.,...&Wang, Hailei.(2016).Conversion of Corn Stalk to Ethanol by One-Step Process using an Alcohol Dehydrogenase Mutant of Phanerochaete chrysosporium.BIORESOURCES,11(4),9940-9955.
MLA	Liu, Lei,et al."Conversion of Corn Stalk to Ethanol by One-Step Process using an Alcohol Dehydrogenase Mutant of Phanerochaete chrysosporium".BIORESOURCES 11.4(2016):9940-9955.