Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice

	Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice
	Zhang, Xu 2,5; Gu, Rui 1,4; Zhang, Ge 3; Tao, Tingting 1,2; Yu, Haibo 5; Liu, Lianqing 5; Dou, Ying 1; Li, Aiping 1; Qin, Jianhua 2
刊名	NEUROCHEMICAL RESEARCH
	2017-05-01
卷号	42 期号:5 页码:1478-1487
关键词	Microfluidic device Microglia Glioma Epithelial-mesenchymal transition Phagocytosis Migration
英文摘要	It has been proven that microglia are involved in both early and late stages of glioma progression and contribute substantially to the tumor mass of gliomas. Because no appropriate in vitro or in vivo investigative approach is available, the dynamic interaction between microglia and gliomas during tumor formation remains unclear. In this study, three types of microfluidic assay were developed to examine the outcomes of the dynamic interaction between microglia and gliomas. Co-migration assay and two-dimensional cell co-culture assay have been used to show that microglial BV-2 cells migrate toward C6 glioma cells and inhibit tumor growth during the early stage of tumorigenesis. However, in three-dimensional cell spheres (three-dimensional cell co-culture assay) that contain a large amount of glioma cells, mimicking the late stage of glioma growth, the phagocytosis of microglia was suppressed, which suggests that glioma cells could reeducate classically activated microglia into a tumor-promoting state at some point during tumor progression. Notably, we found that microglia could contribute to tumor invasion and acquisition of the epithelial-mesenchymal transition phenotype in the glioma microenvironment during the early stage and the late stage of tumor progression. In conclusion, we have developed a potential quantitative method for in vitro study of glioma immunity and provided evidence for the duality of glioma-associated microglia.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]	Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]	ACTIVATED MICROGLIA ; CELLS ; TRANSITION ; MATRIX ; MECHANISMS ; MACROPHAGE ; RESISTANT ; MIGRATION ; INVASION ; SYSTEM
收录类别	SCI
语种	英语
WOS记录号	WOS:000399906000021
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/151975]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Dalian Med Univ, West Sect 9, Dept Physiol, Lvshun South Rd, Dalian 116044, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China 3.Dalian Med Univ, West Sect 9, Dept Immunol, Lvshun South Rd, Dalian 116044, Peoples R China 4.Gen Hosp Chengdu Army, Ctr Lab Dept, Chengdu 610083, Peoples R China 5.Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xu,Gu, Rui,Zhang, Ge,et al. Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice[J]. NEUROCHEMICAL RESEARCH,2017,42(5):1478-1487.
APA	Zhang, Xu.,Gu, Rui.,Zhang, Ge.,Tao, Tingting.,Yu, Haibo.,...&Qin, Jianhua.(2017).Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice.NEUROCHEMICAL RESEARCH,42(5),1478-1487.
MLA	Zhang, Xu,et al."Probing the Bi-directional Interaction Between Microglia and Gliomas in a Tumor Microenvironment on a Microdevice".NEUROCHEMICAL RESEARCH 42.5(2017):1478-1487.