Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses

	Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses
	Zhao, Chunxia 1,2; Li, Lili 1,2; Zhao, Jieyu 1,2; Zhao, Yanni 1,2; Lu, Xin 1,2; Zhou, Zhihui 1,2; Xu, Guowang 1,2
刊名	SCIENTIFIC REPORTS
	2016-11-29
卷号	6
英文摘要	Senescence is the final stage of leaf growth and development. Many different physiological activities occur during this process. A comprehensive metabolomics analysis of tobacco middle leaves at 5 different developmental stages was implemented through multi-platform methods based on liquid chromatography, capillary electrophoresis and gas chromatography coupled with mass spectrometry. In total, 412 metabolites were identified, including pigments, sterols, lipids, amino acids, polyamines, sugars and secondary metabolites. Dramatic metabolic changes were observed. Firstly, membrane degradation and chlorophyll down-regulation occurred after the 50% flower bud stage. Levels of major membrane lipids decreased, including those of the glycolipids in chloroplast thylakoids and phospholipids in membrane envelopes. Clear decreases in free sterols and acylated sterol glucosides were detected along with the accumulation of sterol esters. The accumulation of alkaloids was found. The amino acid levels were significantly decreased, particularly those of N-rich amino acids (glutamine and asparagine), thus reflecting N translocation. Subsequently, the antioxidant system was activated. Sugar alcohols and polyphenols accumulated when the lower leaves turned yellow. These results comprehensively revealed the metabolic changes that occur during tobacco leaf development and senescence under natural conditions.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	LEAF SENESCENCE ; NICOTIANA-TABACUM ; MASS-SPECTROMETRY ; TRANSGENIC TOBACCO ; ARABIDOPSIS ; PLANTS ; STRESS ; BIOSYNTHESIS ; METABOLISM ; STEROLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000388662600004
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/150357]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Chunxia,Li, Lili,Zhao, Jieyu,et al. Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses[J]. SCIENTIFIC REPORTS,2016,6.
APA	Zhao, Chunxia.,Li, Lili.,Zhao, Jieyu.,Zhao, Yanni.,Lu, Xin.,...&Xu, Guowang.(2016).Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses.SCIENTIFIC REPORTS,6.
MLA	Zhao, Chunxia,et al."Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses".SCIENTIFIC REPORTS 6(2016).