Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid

	Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid
	Bai, Fengwu 2,4,5; Zhang, Mingming 2; Zhang, Keyu 4,5; Mehmood, Muhammad Aamer 3,4,5; Zhao, Zongbao Kent 1; Zhao, Xinqing 4,5
刊名	BIORESOURCE TECHNOLOGY
	2017-12-01
卷号	245 页码:1461-1468
关键词	Saccharomyces cerevisiae Acetic acid stress tolerance Zinc sulfate Ady2p Ethanol production
英文摘要	The aim of this work was to study the effects of deleting acetate transporter gene ADY2 on growth and fermentation of Saccharomyces cerevisiae in the presence of inhibitors. Comparative transcriptome analysis revealed that three genes encoding plasma membrane carboxylic acid transporters, especially ADY2, were significantly downregulated under the zinc sulfate addition condition in the presence of acetic acid stress, and the deletion of ADY2 improved growth of S. cerevisiae under acetic acid, ethanol and hydrogen peroxide stresses. Consistently, a concomitant increase in ethanol production by 14.7% in the presence of 3.6 g/L acetic acid was observed in the ADY2 deletion mutant of S. cerevisiae BY4741. Decreased intracellular acetic acid, ROS accumulation, and plasma membrane permeability were observed in the ADY2 deletion mutant. These findings would be useful for developing robust yeast strains for efficient ethanol production. (C) 2017 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	SELF-FLOCCULATING YEAST ; PROGRAMMED CELL-DEATH ; TRANSCRIPTOME ANALYSIS ; LABORATORY EVOLUTION ; IDENTIFICATION ; ZINC ; OVEREXPRESSION ; FERMENTATION ; RESISTANCE ; SUPPLEMENTATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000413038300021
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/150123]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 2.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 3.Govt Coll Univ Faisalabad, Dept Bioinformat & Biotechnol, Bioenergy Res Ctr, Faisalabad 38000, Pakistan 4.Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China 5.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China
推荐引用方式 GB/T 7714	Bai, Fengwu,Zhang, Mingming,Zhang, Keyu,et al. Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid[J]. BIORESOURCE TECHNOLOGY,2017,245:1461-1468.
APA	Bai, Fengwu,Zhang, Mingming,Zhang, Keyu,Mehmood, Muhammad Aamer,Zhao, Zongbao Kent,&Zhao, Xinqing.(2017).Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid.BIORESOURCE TECHNOLOGY,245,1461-1468.
MLA	Bai, Fengwu,et al."Deletion of acetate transporter gene ADY2 improved tolerance of Saccharomyces cerevisiae against multiple stresses and enhanced ethanol production in the presence of acetic acid".BIORESOURCE TECHNOLOGY 245(2017):1461-1468.