Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance

	Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance
	Gao, Meijing 1; Dai, Yan 3,4; Wei, Xiaoli 3,4; Ni, Mi 3,4; Ma, Wei 5; Wang, Xiaofang 3,4; Zhang, Lei 3,4; Peng, Yonggang 3,4; Chen, Shuyuan 3,4; Ding, Lingyun 5
刊名	PLANT BIOTECHNOLOGY JOURNAL
	2017-09-01
卷号	15 期号:9 页码:1204-1213
关键词	genetic engineering RNA interference Bacillus thuringiensis juvenile hormone Helicoverpa armigera sustainability
英文摘要	Transgenic crops producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are extensively cultivated worldwide. To counter rapidly increasing pest resistance to crops that produce single Bt toxins, transgenic plant 'pyramids' producing two or more Bt toxins that kill the same pest have been widely adopted. However, cross-resistance and antagonism between Bt toxins limit the sustainability of this approach. Here we describe development and testing of the first pyramids of cotton combining protection from a Bt toxin and RNA interference (RNAi). We developed two types of transgenic cotton plants producing double-stranded RNA (dsRNA) from the global lepidopteran pest Helicoverpa armigera designed to interfere with its metabolism of juvenile hormone (JH). We focused on suppression of JH acid methyltransferase (JHAMT), which is crucial for JH synthesis, and JH-binding protein (JHBP), which transports JH to organs. In 2015 and 2016, we tested larvae from a Bt-resistant strain and a related susceptible strain of H. armigera on seven types of cotton: two controls, Bt cotton, two types of RNAi cotton (targeting JHAMT or JHBP) and two pyramids (Bt cotton plus each type of RNAi). Both types of RNAi cotton were effective against Bt-resistant insects. Bt cotton and RNAi acted independently against the susceptible strain. In computer simulations of conditions in northern China, where millions of farmers grow Bt cotton as well as abundant non-transgenic host plants of H. armigera, pyramided cotton combining a Bt toxin and RNAi substantially delayed resistance relative to using Bt cotton alone.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Plant Sciences
研究领域[WOS]	Biotechnology & Applied Microbiology ; Plant Sciences
关键词[WOS]	FIELD-EVOLVED RESISTANCE ; WESTERN CORN-ROOTWORM ; DIABROTICA-VIRGIFERA-VIRGIFERA ; BACILLUS-THURINGIENSIS TOXINS ; JUVENILE-HORMONE ; HELICOVERPA-ARMIGERA ; PEST-MANAGEMENT ; CROPS ; GENE ; BOLLWORM
收录类别	SCI
语种	英语
WOS记录号	WOS:000407378100011
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/149840]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Jiangsu, Peoples R China 2.Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA 3.Chinese Acad Sci, State Key Lab Plant Genom, Beijing, Peoples R China 4.Chinese Acad Sci, Natl Ctr Plant Gene Res Beijing, Inst Genet & Dev Biol, Beijing, Peoples R China 5.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Jiangsu Plant Gene Engn Res Ctr, Nanjing, Jiangsu, Peoples R China 6.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Gao, Meijing,Dai, Yan,Wei, Xiaoli,et al. Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance[J]. PLANT BIOTECHNOLOGY JOURNAL,2017,15(9):1204-1213.
APA	Gao, Meijing.,Dai, Yan.,Wei, Xiaoli.,Ni, Mi.,Ma, Wei.,...&Zhu, Zhen.(2017).Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance.PLANT BIOTECHNOLOGY JOURNAL,15(9),1204-1213.
MLA	Gao, Meijing,et al."Next-generation transgenic cotton: pyramiding RNAi and Bt counters insect resistance".PLANT BIOTECHNOLOGY JOURNAL 15.9(2017):1204-1213.