Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles

	Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles
	You, Jinmao1,2,3 ; Qu, Fei 2,3; Liu, Yanqun 2,3; Lao, Haili 4; Wang, Yaping 2,3
刊名	NEW JOURNAL OF CHEMISTRY
	2017-10-07
卷号	41 期号:19 页码:10592-10597
英文摘要	Herein, a new colorimetric strategy based on polymer nanoparticles (PNPs) and Au nanoparticles (AuNPs) is developed to detect heparin (Hep). The cationic PNPs can directly induce the aggregation of AuNPs, which are stabilized by negatively charged coating of citrate ions, and hence the absorption peak of AuNPs moves to 675 nm corresponding with a color change from wine red to blue. However, the negatively charged Hep competes with AuNPs and binds to PNPs, hindering the interaction between PNPs and AuNPs. As a result, the absorption band of AuNPs blue shifts and the color turns to red again. The reaction can be completed within 10 min, and the color variation produced by 150 nM of Hep can be distinguished by the naked eye. In addition, this strategy also shows good selectivity and sensitivity, and the linear range of Hep is from 10 to 400 nM with detection limit of 2.5 nM. Finally, this colorimetric assay has been applied to measure Hep in Hep sodium injection and human serum samples with satisfactory recoveries.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Multidisciplinary
研究领域[WOS]	Chemistry
关键词[WOS]	PEROXIDASE-LIKE ACTIVITY ; INDUCED THROMBOCYTOPENIA ; THROMBOPLASTIN TIME ; INDUCED EMISSION ; QUANTUM DOTS ; SULFATE ; ASSAY ; NANOCOMPOSITES ; NANOCLUSTERS ; SURFACTANT
收录类别	SCI
语种	英语
WOS记录号	WOS:000411768600011
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/9813]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 3.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 4.Binzhou Cent Hosp, Binzhou Med Coll, Dept Clin Lab, Binzhou 256600, Shandong, Peoples R China
推荐引用方式 GB/T 7714	You, Jinmao,Qu, Fei,Liu, Yanqun,et al. Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles[J]. NEW JOURNAL OF CHEMISTRY,2017,41(19):10592-10597.
APA	You, Jinmao,Qu, Fei,Liu, Yanqun,Lao, Haili,&Wang, Yaping.(2017).Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles.NEW JOURNAL OF CHEMISTRY,41(19),10592-10597.
MLA	You, Jinmao,et al."Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles".NEW JOURNAL OF CHEMISTRY 41.19(2017):10592-10597.