A series of novel dibenzothiophene-based two-photon fluorescent probes for cellular nucleus imaging

CORC > 上海光学精密机械研究所 > 中国科学院上海光学精密机械研究所 > 高密度光存储技术实验室

	A series of novel dibenzothiophene-based two-photon fluorescent probes for cellular nucleus imaging
	Chi, Shuheng; Li, Liang; Wu, Yiqun
刊名	Sens. Actuator B-Chem.
	2016
卷号	231 页码:811
通讯作者	chshhen@163.com ; brightlee@siom.ac.cn ; yqwu@siom.ac.cn
英文摘要	In this study, a series of novel dibenzothiophene-based two-photon excited fluorescence (TPEF) probes with excellent nuclear staining ability were prepared and investigated. The one-photon photophysical properties indicated these labels possessed UV absorption wavelength of 390-393 nm and quantum yields of 0.425-0.71. The two-photon photophysical performance exhibited two-photon absorption (TPA) cross-sections of 506-630 GM by excitation of femtosecond laser at a wavelength of 800 nm. 18-30-fold one-photon excited fluorescence (OPEF) enhancement and 300-450% TPEF increment binding with calf thymus DNA in Tris-HC1 buffer solutions displayed obvious fluorescence "turn-on" effect. The co-localization studies showed mean co-localization coefficients of 0.88-0.94 by double-staining with commercial nuclear staining reagent DAPI in 3T3 cells, indicating accurate nuclear localization ability. The confocal fluorescence imaging displayed clear TPEF nuclear imaging. The staining penetration depth of 0-9 mu m exhibited good membrane permeability and three dimensional imaging ability. The observation time of 1800 s and fluorescence decay amplitude of 8-10% within 300 s under persistent irradiation were achieved in TPEF imaging for nuclear labeling. The DNA binding mechanism was discussed as well. (C) 2016 Elsevier B.V. All rights reserved.
收录类别	SCI
资助信息	National Natural Science Foundation of China [61137002, 61178059, 51172253]
WOS记录号	WOS:000374330900094
内容类型	期刊论文
源URL	[http://ir.siom.ac.cn/handle/181231/28226]
专题	上海光学精密机械研究所_高密度光存储技术实验室
作者单位	中国科学院上海光学精密机械研究所
推荐引用方式 GB/T 7714	Chi, Shuheng,Li, Liang,Wu, Yiqun. A series of novel dibenzothiophene-based two-photon fluorescent probes for cellular nucleus imaging[J]. Sens. Actuator B-Chem.,2016,231:811.
APA	Chi, Shuheng,Li, Liang,&Wu, Yiqun.(2016).A series of novel dibenzothiophene-based two-photon fluorescent probes for cellular nucleus imaging.Sens. Actuator B-Chem.,231,811.
MLA	Chi, Shuheng,et al."A series of novel dibenzothiophene-based two-photon fluorescent probes for cellular nucleus imaging".Sens. Actuator B-Chem. 231(2016):811.