Bladder function in mice with inducible smooth muscle-specific deletion of the manganese superoxide dismutase gene

doi:10.1152/ajpcell.00046.2015

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	Bladder function in mice with inducible smooth muscle-specific deletion of the manganese superoxide dismutase gene
	Liu, GM; Elrashidy, RA; Xiao, N; Kavran, M; Huang, YX; Tao, MF; Powell, CT; Kim, E; Sadeghi, G; Mohamed, HE
刊名	AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
	2015-08-01
卷号	309 期号:3 页码:C169-C178
关键词	oxidative stress inducible cre-loxp recombination
ISSN号	0363-6143
DOI	10.1152/ajpcell.00046.2015
文献子类	Article
英文摘要	Manganese superoxide dismutase (MnSOD) is considered a critical component of the antioxidant systems that protect against oxidative damage. We are interested in the role of oxidative stress in bladder detrusor smooth muscle (SM) in different disease states. In this study, we generated an inducible, SM-specific Sod2(-/-) mouse model to investigate the effects of MnSOD depletion on the function of the bladder. We crossbred floxed Sod2 (Sod2(lox/lox)) mice with mice containing heterozygous knock-in of a gene encoding a tamoxifen-activated Cre recombinase in the SM22 alpha promoter locus [SM-CreER(T2)(ki)(Cre/+)]. We obtained Sod2(lox/lox), SM-CreER(T2)(ki)(Cre/+) mice and injected 8-wk-old males with 4-hydroxytamoxifen to induce Cre-mediated excision of the floxed Sod2 allele. Twelve weeks later, SM-specific deletion of Sod2 and depletion of MnSOD were confirmed by polymerase chain reaction, immunoblotting, and immunohistochemistry. SM-specific Sod2(-/-) mice exhibited normal growth with no gross abnormalities. A significant increase in nitrotyrosine concentration was found in bladder SM tissue of SM-specific Sod2(-/-) mice compared with both wild-type mice and Sod2(+/+), SM-CreER(T2)(ki)(Cre/+) mice treated with 4-hydroxytamoxifen. Assessment of 24-h micturition in SM-specific Sod2(-/-) mice revealed significantly higher voiding frequency compared with both wild-type and SM-specific Cre controls. Conscious cystometry revealed significantly shorter intercontraction intervals and lower functional bladder capacity in SM-specific Sod2(-/-) mice compared with wild-type mice. This novel model can be used for exploring the mechanistic role of oxidative stress in organs rich in SM in different pathological conditions.
学科主题	Cell Biology ; Physiology
出版地	BETHESDA
项目编号	National Institute of Diabetes and Digestive and Kidney Diseases [U01-DK-076162, P20-DK-090871, R01-DK-083733] ; Egyptian Cultural Affairs and Missions Sector
语种	英语
WOS记录号	WOS:000358946700005
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179380]
专题	第二临床医学院_期刊论文
通讯作者	Daneshgari, F (reprint author), Case Western Reserve Univ, Sch Med, Dept Urol, 11100 Euclid Ave, Cleveland, OH 44106 USA.
推荐引用方式 GB/T 7714	Liu, GM,Elrashidy, RA,Xiao, N,et al. Bladder function in mice with inducible smooth muscle-specific deletion of the manganese superoxide dismutase gene[J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,2015,309(3):C169-C178.
APA	Liu, GM.,Elrashidy, RA.,Xiao, N.,Kavran, M.,Huang, YX.,...&Daneshgari, F .(2015).Bladder function in mice with inducible smooth muscle-specific deletion of the manganese superoxide dismutase gene.AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY,309(3),C169-C178.
MLA	Liu, GM,et al."Bladder function in mice with inducible smooth muscle-specific deletion of the manganese superoxide dismutase gene".AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 309.3(2015):C169-C178.