High-resolution melting technology for detecting genetic polymorphisms of IL-1beta and IL-1Ra in patients with coronary artery disease

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	High-resolution melting technology for detecting genetic polymorphisms of IL-1beta and IL-1Ra in patients with coronary artery disease
	Qi, Xiaoming; Bai, Feng; You, Chongge; Guo, Xinling; Shi, Lei; Hu, Zhibin
刊名	细胞与分子免疫学杂志
	2015-06
卷号	31 期号:6 页码:816-820
关键词	高分辨率融解曲线分析技术白细胞介素1β 白细胞介素1受体拮抗剂单核苷酸多态性冠心病
ISSN号	1007-8738
其他题名	高分辨率融解曲线技术检测冠心病患者IL-1β和IL-1Ra基因多态性
文献子类	Article
英文摘要	OBJECTIVE: To develop a method of high-resolution melting curve analysis (HRM) for investigating the association of single nucleotide polymorphisms (SNPs) of IL-1beta and IL-1Ra genes with susceptibility to coronary heart disease (CHD) and serological indexes in CHD.METHODS: PCR-HRM assays were established for detecting four SNPs (IL-1B-511C>T, IL-1B-31C>T, IL-1B+3954C>T and IL-1RNT>C) in 260 patients with CHD and 274 healthy controls. A case-control study was performed for analyzing the relationships of the four SNPs with CHD risk and serological indexes.RESULTS: No significant statistical difference was observed between the four SNPs and the susceptibility to CHD, however, the result of gender stratification showed that there was the statistical differences in the male IL-1B-31C>T and the female IL-1B+3954C>T allele frequencies (P=0.039, 0.032, respectively). The result of haplotype analysis of the four SNPs showed that haplotype of IL-1B-511T/IL-1B-31C/IL-1B+3954C/IL-1RN T (TCCT) had a significant correlation with obviously increased CHD risk, but without statistical difference (OR=1.217, 95%CI: 0.950-1.559, P=0.123), while those of CTCC and TTCT had significant correlations with decreased CHD risk (OR=0.612, 95%CI: 0.376-0.994, P=0.046; OR=0.365, 95%CI: 0.154-0.862, P=0.017). No association was found between the four SNPs and the serological indexes in CHD subjects.CONCLUSION: The developed PCR-HRM detection assays based on HRM technology has been verified by direct sequencing and clinical specimen detection to be a rapid and homogeneous detection method for genotyping of the four SNPs. The SNPs of IL-1B-31C>T, IL-1B+3954C>T and the haplotypes of CTCC and TTCT are associated with the susceptibility to CHD in Chinese Han population of Lanzhou region.
学科主题	Genetics & Heredity ; Cardiovascular System & Cardiology ; Biochemistry & Molecular Biology ; Immunology ; Physics (Provided By Thomson Reuters)
出版地	XI'AN
资助项目	春晖计划 ; 甘肃省技术研究与开发专项计划
项目编号	教育部春晖计划项目 ; 甘肃省技术研究与开发专项
语种	中文
CSCD记录号	CSCD:5412985
资助机构	MOE ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179361]
专题	第二临床医学院_期刊论文
推荐引用方式 GB/T 7714	Qi, Xiaoming,Bai, Feng,You, Chongge,et al. High-resolution melting technology for detecting genetic polymorphisms of IL-1beta and IL-1Ra in patients with coronary artery disease[J]. 细胞与分子免疫学杂志,2015,31(6):816-820.
APA	Qi, Xiaoming,Bai, Feng,You, Chongge,Guo, Xinling,Shi, Lei,&Hu, Zhibin.(2015).High-resolution melting technology for detecting genetic polymorphisms of IL-1beta and IL-1Ra in patients with coronary artery disease.细胞与分子免疫学杂志,31(6),816-820.
MLA	Qi, Xiaoming,et al."High-resolution melting technology for detecting genetic polymorphisms of IL-1beta and IL-1Ra in patients with coronary artery disease".细胞与分子免疫学杂志 31.6(2015):816-820.