Hesperidin Alleviates Oxidative Stress and Upregulates the Multidrug Resistance Protein 2 in Isoniazid and Rifampicin-Induced Liver Injury in Rats

doi:10.1002/jbt.21799

CORC > 兰州大学 > 兰州大学 > 第一临床医学院 > 期刊论文

	Hesperidin Alleviates Oxidative Stress and Upregulates the Multidrug Resistance Protein 2 in Isoniazid and Rifampicin-Induced Liver Injury in Rats
	Zhang, GQ; Zhu, JF; Zhou, Y; Wei, YH; Xi, LL; Qin, HY; Rao, Z; Han, M; Ma, YR; Wu, XA
刊名	JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
	2016-07
卷号	30 期号:7 页码:342-349
关键词	Isoniazid Rifampicin Hesperidin Oxidative stress Multidrug resistance proteins
ISSN号	1095-6670
DOI	10.1002/jbt.21799
文献子类	Article
英文摘要	Isoniazid (INH) and rifampicin (RFP), two front-line drugs used in tuberculosis therapy, may lead to seriously hepatotoxicity. The current study was carried out to investigate the hepatoprotective effects of hesperidin against INH- and RFP-induced oxidative damage. The liver injury animal model of rats was induced by INH (75 mg/kg) and RFP (150 mg/kg) coadministration for 7 days, and hesperidin, at the dose of 50, 100, and 200 mg/kg, was orally administered to rats 2 h before INH and RFP administration. The biochemical and pathologic examinations were performed after rats were sacrificed. Moreover, the serum and liver glutathione (GSH), glutathione disulfide (GSSG), malondialdehyde (MDA), GSH peroxidase, and GSSG reductase were determined by test kits, and the expression of multidrug resistance proteins 2 (Mrp2) was determined by Western blotting and immunohistochemistry. The results showed that hesperidin significantly alleviated liver injury as indicated by the decreased levels of ALT, AST, bilirubin, total bile acid, and glutathione peroxidase and the increased levels of the GSH/GSSG ratio and the expression of Mrp2. Moreover, hesperidin could effectively reduce the pathological tissue damage. These results indicate that hesperidin can attenuate INH-and RFP-induced oxidative damage, and the underlying mechanism may have correlation with its effect on the upregulation of Mrp2. (C) 2016 Wiley Periodicals, Inc.
学科主题	Biochemistry & Molecular Biology ; Toxicology
出版地	HOBOKEN
资助项目	国家自然科学基金项目
项目编号	National Natural Science Foundation of China [81373494] ; First Hospital of Lanzhou University Foundation [ldyyyn2013-09]
语种	英语
WOS记录号	WOS:000382575100003
资助机构	NSFC ; LZU
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/178659]
专题	第一临床医学院_期刊论文
通讯作者	Wu, XA (reprint author), Lanzhou Univ, Hosp 1, Dept Pharm, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Zhang, GQ,Zhu, JF,Zhou, Y,et al. Hesperidin Alleviates Oxidative Stress and Upregulates the Multidrug Resistance Protein 2 in Isoniazid and Rifampicin-Induced Liver Injury in Rats[J]. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,2016,30(7):342-349.
APA	Zhang, GQ.,Zhu, JF.,Zhou, Y.,Wei, YH.,Xi, LL.,...&Wu, XA .(2016).Hesperidin Alleviates Oxidative Stress and Upregulates the Multidrug Resistance Protein 2 in Isoniazid and Rifampicin-Induced Liver Injury in Rats.JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,30(7),342-349.
MLA	Zhang, GQ,et al."Hesperidin Alleviates Oxidative Stress and Upregulates the Multidrug Resistance Protein 2 in Isoniazid and Rifampicin-Induced Liver Injury in Rats".JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY 30.7(2016):342-349.