Expression Profile of Apoptosis Related Genes and Radio-sensitivity of Prostate Cancer Cells

doi:10.1269/jrr.10190

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	Expression Profile of Apoptosis Related Genes and Radio-sensitivity of Prostate Cancer Cells
	He, ZH; Zhang, Y; Mehta, SK; Pierson, DL; Wu, HL; Rohde, LH; Zhang, Y (reprint author), Wyle Integrated Sci & Engn Grp, Houston, TX 77058 USA.
刊名	JOURNAL OF RADIATION RESEARCH
	2011-11
卷号	52 期号:6 页码:743-751
关键词	Prostate cancer cells X-rays Monensin Apoptosis Gene expression
ISSN号	0449-3060
DOI	10.1269/jrr.10190
文献子类	Article
英文摘要	Radio-resistant or recurrent prostate cancer represents a serious health risk for approximately 20%-30% of patients treated with primary radiation therapy for clinically localized prostate cancer. In the present study, we investigated the expression profiles of 84 genes involved in various apoptosis pathways in two prostate cancer cell lines LNCaP (P53+ and AR+) and PC3 (P53- and AR-). We also studied the effect of monensin, an apoptosis inducing reagent, in X-ray-induced cell killing. Comparison of gene expressions between unirradiated LNCaP and PC3 cells revealed distinguished gene expression patterns. The data showed a significantly higher expression level of genes involved in the caspase/card family and the TNF ligand/receptor family in PC3 cells, whereas, LNCaP cells exhibited higher expressions in the p53 related genes. At 2 and 4 hrs post a 10 Gy X-ray exposure, changes of gene expressions were detected in a significant fraction of the genes in LNCaP cells, but no significant changes were found in PC3 cells. There was no significant apoptosis-inducing effect of X-rays (up to 10 Gy) in both cell lines; however, monensin was shown to be effective in inducing apoptosis in LNCaP, but not in PC3 cells. In addition, the effect of combined treatment of monensin and X-rays in LNCaP cells appeared to be synergistic. Our results suggest that monensin may be effective for both cancer cell killing and radiosensitizing, and the different expression profiles in apoptosis related genes in cancer cells may be correlated with their sensitivity to apoptosis inducing reagents.
学科主题	Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging
出版地	CHIBA
项目编号	University of Houston, Institute for Space Systems Operations (ISSO)
语种	英语
WOS记录号	WOS:000297565800008
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/125927]
专题	第一临床医学院_期刊论文
通讯作者	Zhang, Y (reprint author), Wyle Integrated Sci & Engn Grp, Houston, TX 77058 USA.
推荐引用方式 GB/T 7714	He, ZH,Zhang, Y,Mehta, SK,et al. Expression Profile of Apoptosis Related Genes and Radio-sensitivity of Prostate Cancer Cells[J]. JOURNAL OF RADIATION RESEARCH,2011,52(6):743-751.
APA	He, ZH.,Zhang, Y.,Mehta, SK.,Pierson, DL.,Wu, HL.,...&Zhang, Y .(2011).Expression Profile of Apoptosis Related Genes and Radio-sensitivity of Prostate Cancer Cells.JOURNAL OF RADIATION RESEARCH,52(6),743-751.
MLA	He, ZH,et al."Expression Profile of Apoptosis Related Genes and Radio-sensitivity of Prostate Cancer Cells".JOURNAL OF RADIATION RESEARCH 52.6(2011):743-751.