Matrine alters microRNA expression profiles in SGC-7901 human gastric cancer cells

doi:10.3892/or_xxxxxxxx

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	Matrine alters microRNA expression profiles in SGC-7901 human gastric cancer cells
	Li, HL; Xie, SP; Liu, XJ; Wu, HY; Lin, XY; Gu, J; Wang, HP; Duan, YQ; Duan, YQ (reprint author), Gansu Tradit Chinese Med Univ, Sch Basic Med Sci, 35 Dingxi Rd, Lanzhou 730000, Gansu, Peoples R China.
刊名	ONCOLOGY REPORTS
	2014-11
卷号	32 期号:5 页码:2118-2126
关键词	miRNA microarray profile matrine gastric cancer
ISSN号	1021-335X
DOI	10.3892/or_xxxxxxxx
文献子类	Article
英文摘要	Matrine, a major alkaloid extracted from Sophora flavescens, has been reported to possess antitumor properties in several types of cancers, including gastric cancer. However, its mechanisms of action on gastric cancer remain poorly understood. Dysregulation of microRNAs, a class of small, non-coding, regulatory RNA molecules involved in gene expression, is strongly correlated with cancer. The aim of the present study was to demonstrate that matrine treatment altered miRNA expression in SGC7901 cells. Using miRCURYTM microarray analysis, we identified 128 miRNAs substantially exhibiting >2-fold expression changes in matrine-treated cells relative to their expression levels in untreated cells. RT-qPCR was used to show that the levels of 8 miRNAs whose target genes were clustered in the cell cycle pathway increased, while levels of 14 miRNAs whose target genes were clustered in the MAPK signaling pathway decreased. These results were consistent with those from the miRNA microarray experiment. Bioinformatical analysis revealed that the majority of 57 identified enrichment pathways were highly involved in tumorigenesis. In conclusion, the results demonstrated that matrine induces considerable changes in the miRNA expression profiles of SGC7901 cells, suggesting miRNA microarray combined with RT-qPCR validation and bioinformatical analysis provide a novel and promising approach to identify anticancer targets and the mechanisms of matrine involved.
学科主题	Oncology
出版地	ATHENS
项目编号	Key Laboratory of TCM Pharmacology and Toxicology of Gansu Province [ZDSYS-KJ-2012-003, ZDSYS-KJ-2013-009]
语种	英语
WOS记录号	WOS:000342714000046
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/125855]
专题	第一临床医学院_期刊论文
通讯作者	Duan, YQ (reprint author), Gansu Tradit Chinese Med Univ, Sch Basic Med Sci, 35 Dingxi Rd, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Li, HL,Xie, SP,Liu, XJ,et al. Matrine alters microRNA expression profiles in SGC-7901 human gastric cancer cells[J]. ONCOLOGY REPORTS,2014,32(5):2118-2126.
APA	Li, HL.,Xie, SP.,Liu, XJ.,Wu, HY.,Lin, XY.,...&Duan, YQ .(2014).Matrine alters microRNA expression profiles in SGC-7901 human gastric cancer cells.ONCOLOGY REPORTS,32(5),2118-2126.
MLA	Li, HL,et al."Matrine alters microRNA expression profiles in SGC-7901 human gastric cancer cells".ONCOLOGY REPORTS 32.5(2014):2118-2126.