Cold Plasma Therapy of a Tooth Root Canal Infected with Enterococcus faecalis Biofilms In Vitro

doi:10.1016/j.joen.2012.08.017

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	Cold Plasma Therapy of a Tooth Root Canal Infected with Enterococcus faecalis Biofilms In Vitro
	Pan, J; Sun, K; Liang, YD; Sun, P; Yang, XH; Wang, J; Zhang, J; Zhu, WD; Fang, J; Becker, KH
刊名	JOURNAL OF ENDODONTICS
	2013-01
卷号	39 期号:1 页码:105-110
关键词	Biofilm cold plasma disinfection Enterococcus faecalis root canal
ISSN号	0099-2399
DOI	10.1016/j.joen.2012.08.017
文献子类	Article
英文摘要	Introduction: Complete sterilization of an infected root canal is an important challenge in endodontic treatment. Traditional methods often cannot achieve high-efficiency sterilization because of the complexity of the root canal system. The objective of the study was to investigate in vitro the feasibility of using a cold plasma treatment of a root canal infected with Enterococcus faecalis biofilms. Methods: Seventy single-root teeth infected with E. faecalis biofilms were divided into 7 groups. Group 1 served as the negative control group (no treatment), and group 7 was the positive control group with teeth treated with calcium hydroxide intracanal medication for 7 days. Groups 2 to 6 included teeth treated by cold plasma for 2, 4, 6, 8, and 10 minutes, respectively. The disinfection of the E. faecalis biofilm was evaluated by colony-forming unit (CFU) counting. Scanning electron microscopy was used to evaluate the structural changes of the E. faecalis biofilm before and after plasma treatment. Confocal scanning laser microscopy was used to investigate the vitality of the microorganisms in the biofilm before and-after plasma treatment. Results: A significant decrease in the number of CFUs was observed after prolonged cold plasma treatment (based on the statistical analysis of the teeth in groups 2-6). Compared with the positive control group, cold plasma treatment of 8 or 10 minutes (groups 5 and 6) had a significantly higher antimicrobial efficacy (P < .05). The scanning electron microscopic analysis showed that the bacteria membrane was ruptured, and the structure of the biofilm was fully destroyed by the plasma. Confocal scanning laser microscopic studies indicated that the plasma treatment induced E. faecalis death and destruction of the biofilm. Conclusions: The cold plasma-had a high efficiency in disinfecting the E. faecalis biofilms in in vitro dental root Canal treatment. (J Endod 2013;39:105-110)
学科主题	Dentistry, Oral Surgery & Medicine
出版地	NEW YORK
资助项目	国家重点基础研究发展计划以及国家重大科学研究计划（973计划） ; 国家国际科技合作专项
项目编号	College of Engineering, Peking University ; Medicine School of Peking University ; Bioelectrics Inc (Raleigh, NC) ; MST Program of International Science and Technology Cooperation [2009DFB30370] ; National Basic Research Program [2007CB935602] ; Electro-Energetic Physics Program of the United States Air Force Office of Scientific Research [FA9550-08-1-0332]
语种	英语
WOS记录号	WOS:000313378900020
资助机构	MOST
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/122102]
专题	口腔医学院_期刊论文
通讯作者	Zhang, J (reprint author), Peking Univ, Coll Engn, Acad Adv Interdisciplinary Studies, 5 Yiheyuan Rd, Beijing 100871, Peoples R China.
推荐引用方式 GB/T 7714	Pan, J,Sun, K,Liang, YD,et al. Cold Plasma Therapy of a Tooth Root Canal Infected with Enterococcus faecalis Biofilms In Vitro[J]. JOURNAL OF ENDODONTICS,2013,39(1):105-110.
APA	Pan, J.,Sun, K.,Liang, YD.,Sun, P.,Yang, XH.,...&Zhang, J .(2013).Cold Plasma Therapy of a Tooth Root Canal Infected with Enterococcus faecalis Biofilms In Vitro.JOURNAL OF ENDODONTICS,39(1),105-110.
MLA	Pan, J,et al."Cold Plasma Therapy of a Tooth Root Canal Infected with Enterococcus faecalis Biofilms In Vitro".JOURNAL OF ENDODONTICS 39.1(2013):105-110.