A microbial transformation using Bacillus subtilis B7-S to produce natural vanillin from ferulic acid

doi:10.1038/srep20400

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	A microbial transformation using Bacillus subtilis B7-S to produce natural vanillin from ferulic acid
	Chen, P; Yan, L; Wu, ZR; Li, SY; Bai, ZT; Yan, XJ; Wang, NB; Liang, N; Li, HY; Li, HY (reprint author), Lanzhou Univ, Sch Pharm, Donggang West Rd 199, Lanzhou 730020, Peoples R China.
刊名	SCIENTIFIC REPORTS
	2016-02-04
卷号	6
ISSN号	2045-2322
DOI	10.1038/srep20400
文献子类	Article
英文摘要	Bacillus subtilis strain B7-S screened from18 strains is an aerobic, endospore-forming, model organism of Gram-positive bacteria which is capable to form vanillin during ferulic acid bioconversion. The bioconversion of ferulic acid to vanillin by Bacillus subtilis B7-S (B. subtilis B7-S) was investigated. Based on our results, the optimum bioconversion conditions for the production of vanillin by B. subtilis B7-S can be summarized as follows: temperature 35 degrees C; initial pH 9.0; inoculum volume 5%; ferulic acid concentration 0.6 g/L; volume of culture medium 20%; and shaking speed 200 r/min. Under these conditions, several repeated small-scale batch experiments showed that the maximum conversion efficiency was 63.30% after 3 h of bioconversion. The vanillin products were confirmed by spectral data achieved from UV-vis, inductively coupled plasma atomic emission spectroscope (ICP-AES) and Fourier transform infrared spectrometer (FT-IR) spectra. Scanning electron microscopy (SEM) and transmission electron spectroscopy (TEM) results confirmed that the cell surface of B. subtilis plays a role in the induction of ferulic acid tolerance. These results demonstrate that B. subtilis B7-S has the potential for use in vanillin production through bioconversion of ferulic acid.
学科主题	Science & Technology - Other Topics
出版地	LONDON
资助项目	国家自然科学基金项目 ; 中央高校基本科研业务费专项资金 ; 甘肃省杰出青年基金计划 ; 甘肃省技术研究与开发专项计划 ; 兰州市科技局资助项目
项目编号	Gansu Province Science Foundation for Distinguished Young Scholars [1308RJDA014] ; Longyuan Support Project for Young Creative Talents [GANZUTONGZI [2014]] ; Technology Program of Gansu Province [1205TCYA034] ; Technology Program of Lanzhou City [2013-4-115, 2015-3-93] ; Fundamental Research Funds for the Central Universities of China [lzujbky-2015-57] ; Natural Science Foundation of Gansu Province [1208RJZA219] ; Program of Science and Technology Innovation Teams Building in Heilongjiang Province [2012TD006] ; Scientific Research Fund of Heilongjiang Provincial Education Department [12511361]
语种	英语
WOS记录号	WOS:000369271800001
资助机构	NSFC ; LZU ; GSSTD ; LZSTB
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179573]
专题	药学院_期刊论文
通讯作者	Li, HY (reprint author), Lanzhou Univ, Sch Pharm, Donggang West Rd 199, Lanzhou 730020, Peoples R China.
推荐引用方式 GB/T 7714	Chen, P,Yan, L,Wu, ZR,et al. A microbial transformation using Bacillus subtilis B7-S to produce natural vanillin from ferulic acid[J]. SCIENTIFIC REPORTS,2016,6.
APA	Chen, P.,Yan, L.,Wu, ZR.,Li, SY.,Bai, ZT.,...&Li, HY .(2016).A microbial transformation using Bacillus subtilis B7-S to produce natural vanillin from ferulic acid.SCIENTIFIC REPORTS,6.
MLA	Chen, P,et al."A microbial transformation using Bacillus subtilis B7-S to produce natural vanillin from ferulic acid".SCIENTIFIC REPORTS 6(2016).