Effect of Apigenin on Acrylontrile-Induced Lipid Peroxidation and DNA Damage in Sperm of Male Rats

doi:10.7524/AJE.1673-5897.20150601003

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	Effect of Apigenin on Acrylontrile-Induced Lipid Peroxidation and DNA Damage in Sperm of Male Rats
	Chen Junyi; Zhao Qianlong; Zhang Jie; Chang Ruixia; Zhao Xiaofei; Dang Yuhui; Li Zhilan
刊名	生态毒理学报
	2015-12
卷号	10 期号:6 页码:166-172
关键词	芹菜素丙烯腈精子大鼠脂质过氧化 DNA损伤彗星实验
ISSN号	1673-5897
DOI	10.7524/AJE.1673-5897.20150601003
其他题名	芹菜素对丙烯腈引起大鼠精子脂质过氧化和DNA损伤的影响
文献子类	Article
英文摘要	In this study, the effect of apigenin (AP) on acrylontrile-(ACN) induced lipid peroxidation and DNA damage of sperm in male rats were examined and the possible mechanisms were discussed. 50 healthy adult male Sprague-Dawley rats were randomly divided into five groups: control group (corn oil), ACN group (50 mg.k(-1) ACN), low-dose AP group (50 mg.kg(-1) ACN+234 mg.kg(-1) AP), high-dose AP group (50 mg.kg(-1) ACN+468 mg.kg(-1) AP) and NAC group (50 mg.kg(-1) ACN+300 mg.kg(-1) NAC). The rats were intragastrically administered daily with 5 mL.kg(-1) (boby weight) for 13 weeks, 6 days per week. The sperm samples were collected for the determination of the content of reactive oxygen species (ROS) and malondialdehyde (MDA), the activity of superoxide dismutase (SOD), and the DNA damage. The result showed in comparison with control group, the contents of ROS and MDA, the tail DNA%, tail length, tail moment and Olive tail moment were increased significantly in ACN group, low-dose AP group, high-dose AP group and NAC group, while the activity of SOD was decreased significantly in these groups (P < 0.05). In contrast, the difference among low-dose AP group, high-dose AP group, NAC group and ACN group had no statistical significance (P > 0.05). These data indicate that regardless the presence of AP and/or NAC, ACN could induce sperm lipid peroxidation and DNA damage in male rats.
学科主题	Biochemistry & Molecular Biology ; Reproductive Biology
出版地	BEIJING
资助项目	中央高校基本科研业务费专项资金
项目编号	中央高校基本科研业务专项资金项目
语种	中文
CSCD记录号	CSCD:5649726
资助机构	LZU
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179619]
专题	公共卫生学院_期刊论文
推荐引用方式 GB/T 7714	Chen Junyi,Zhao Qianlong,Zhang Jie,et al. Effect of Apigenin on Acrylontrile-Induced Lipid Peroxidation and DNA Damage in Sperm of Male Rats[J]. 生态毒理学报,2015,10(6):166-172.
APA	Chen Junyi.,Zhao Qianlong.,Zhang Jie.,Chang Ruixia.,Zhao Xiaofei.,...&Li Zhilan.(2015).Effect of Apigenin on Acrylontrile-Induced Lipid Peroxidation and DNA Damage in Sperm of Male Rats.生态毒理学报,10(6),166-172.
MLA	Chen Junyi,et al."Effect of Apigenin on Acrylontrile-Induced Lipid Peroxidation and DNA Damage in Sperm of Male Rats".生态毒理学报 10.6(2015):166-172.