Sulforaphane Attenuates Muscle Inflammation in Dystrophin-deficient mdx Mice via NF-E2-related Factor 2 (Nrf2)-mediated Inhibition of NF-kappa B Signaling Pathway

doi:10.1074/jbc.M115.655019

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	Sulforaphane Attenuates Muscle Inflammation in Dystrophin-deficient mdx Mice via NF-E2-related Factor 2 (Nrf2)-mediated Inhibition of NF-kappa B Signaling Pathway
	Sun, CC; Li, SJ; Yang, CL; Xue, RL; Xi, YY; Wang, L; Zhao, QL; Li, DJ; Li, DJ (reprint author), Wuhan Univ, Dept Occupat & Environm Hlth, Sch Publ Hlth, 115N Donghu Rd, Wuhan 430071, Peoples R China.
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2015-07-17
卷号	290 期号:29 页码:17784-17795
ISSN号	0021-9258
DOI	10.1074/jbc.M115.655019
其他题名	Sulforaphane attenuates muscle inflammation in dystrophin-deficient mdx mice via NF-E2-related factor 2 (Nrf2)-mediated inhibition of NF-κB signaling pathway
文献子类	Article
英文摘要	Inflammation is widely distributed in patients with Duchenne muscular dystrophy and ultimately leads to progressive deterioration of muscle function with chronic muscle damage, oxidative stress, and reduced oxidative capacity. NF-E2-related factor 2 (Nrf2) plays a critical role in defending against inflammation in different tissues via activation of phase II enzyme heme oxygenase-1 and inhibition of the NF-kappa B signaling pathway. However, the role of Nrf2 in the inflammation of dystrophic muscle remains unknown. To determine whether Nrf2 may counteract inflammation in dystrophic muscle, we treated 4-week-old male mdx mice with the Nrf2 activator sulforaphane (SFN) by gavage (2 mg/kg of body weight/day) for 4 weeks. The experimental results demonstrated that SFN treatment increased the expression of muscle phase II enzyme heme oxygenase-1 in an Nrf2-dependent manner. Inflammation in mice was reduced by SFN treatment as indicated by decreased infiltration of immune cells and expression of the inflammatory cytokine CD45 and proinflammatory cytokines tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6 in the skeletal muscles of mdx mice. In addition, SFN treatment also decreased the expression of NF-kappa B(p65) and phosphorylated I kappa B kinase-alpha as well as increased inhibitor of kappa B-alpha expression in mdx mice in an Nrf2-dependent manner. Collectively, these results show that SFN-induced Nrf2 can alleviate muscle inflammation in mdx mice by inhibiting the NF-kappa B signaling pathway.
学科主题	Biochemistry & Molecular Biology
出版地	BETHESDA
资助项目	国家自然科学基金项目
项目编号	National Natural Science Foundation of China [81271943] ; Fundamental Research Funds for the Central Universities [2015305020202]
语种	英语
WOS记录号	WOS:000358511700013
资助机构	NSFC
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179610]
专题	公共卫生学院_期刊论文
通讯作者	Li, DJ (reprint author), Wuhan Univ, Dept Occupat & Environm Hlth, Sch Publ Hlth, 115N Donghu Rd, Wuhan 430071, Peoples R China.
推荐引用方式 GB/T 7714	Sun, CC,Li, SJ,Yang, CL,et al. Sulforaphane Attenuates Muscle Inflammation in Dystrophin-deficient mdx Mice via NF-E2-related Factor 2 (Nrf2)-mediated Inhibition of NF-kappa B Signaling Pathway[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2015,290(29):17784-17795.
APA	Sun, CC.,Li, SJ.,Yang, CL.,Xue, RL.,Xi, YY.,...&Li, DJ .(2015).Sulforaphane Attenuates Muscle Inflammation in Dystrophin-deficient mdx Mice via NF-E2-related Factor 2 (Nrf2)-mediated Inhibition of NF-kappa B Signaling Pathway.JOURNAL OF BIOLOGICAL CHEMISTRY,290(29),17784-17795.
MLA	Sun, CC,et al."Sulforaphane Attenuates Muscle Inflammation in Dystrophin-deficient mdx Mice via NF-E2-related Factor 2 (Nrf2)-mediated Inhibition of NF-kappa B Signaling Pathway".JOURNAL OF BIOLOGICAL CHEMISTRY 290.29(2015):17784-17795.