Global Levels of Histone Modifications in Peripheral Blood Mononuclear Cells of Subjects with Exposure to Nickel

doi:10.1289/ehp.1104140

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	Global Levels of Histone Modifications in Peripheral Blood Mononuclear Cells of Subjects with Exposure to Nickel
	Arita, A; Niu, JP; Qu, QS; Zhao, NJ; Ruan, Y; Nadas, A; Chervona, Y; Wu, F; Sun, H; Hayes, RB
刊名	ENVIRONMENTAL HEALTH PERSPECTIVES
	2012-02
卷号	120 期号:2 页码:198-203
关键词	epigenetics H3K4 trimethylation H3K9 acetylation H3K9 dimethylation histone modifications interindividual variation intraindividual variation nickel nickel refinery workers
ISSN号	0091-6765
DOI	10.1289/ehp.1104140
文献子类	Article
英文摘要	BACKGROUND: Occupational exposure to nickel (Ni) is associated with an increased risk for lung and nasal cancers. Ni compounds exhibit weak mutagenic activity, cause gene amplification, and disrupt cellular epigenetic homeostasis. However, the Ni-induced changes in global histone modification levels have only been tested in vitro. OBJECTIVE: This study was conducted in a Chinese population to determine whether occupational exposure to Ni is associated with alterations of global histone modification levels and to evaluate the inter- and intraindividual variance of global histone modification levels. METHOD: Forty-five subjects with occupational exposure to Ni and 75 referents were recruited. Urinary Ni and global H3K4 trimethylation, H3K9 acetylation, and H3K9 dimethylation levels were measured in peripheral blood mononuclear cells (PBMCs) of subjects. RESULTS: H3K4me3 was elevated in Ni-exposed subjects (0.25% +/- 0.11%) compared with referents (0.15% +/- 0.04%; p = 0.0004), and H3K9me2 was decreased (Ni-exposed subjects, 0.11% +/- 0.05%; referents, 0.15% +/- 0.04%; p = 0.003). H3K4me3 was positively (r = 0.4,p = 0.0008) and H3K9ac was negatively (r = 0.1, p = 0.01) associated with urinary Ni. Interindividual variances of H3K4me3, H3K9ac, and H3K9me2 were larger compared with intraindividual variance in both exposure test groups, resulting in reliability coefficients (an estimate of consistency of a set of measurements) of 0.60, 0.67, and 0.79 for H3K4me3, H3K9ac, and H3K9me2, respectively, for Ni-exposed subjects and of 0.75, 0.74, and 0.97, respectively, for referent subjects. CONCLUSION: The results of this study indicate that occupational exposure to Ni is associated with alterations of global histone modification levels and that measurements of global levels of histone modifications are relatively stable over time in human PBMCs.
学科主题	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
出版地	RES TRIANGLE PK
项目编号	National Institute of Environmental Health Sciences [E5000260, ES010344, ES014454, ES005512] ; National Cancer Institute [CA 16087] ; National Center for Research Resources [RR029893]
语种	英语
WOS记录号	WOS:000299915400021
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121805]
专题	公共卫生学院_期刊论文
通讯作者	Costa, M (reprint author), NYU, Dept Environm Med, 57 Old Forge Rd, Tuxedo Pk, NY 10987 USA.
推荐引用方式 GB/T 7714	Arita, A,Niu, JP,Qu, QS,et al. Global Levels of Histone Modifications in Peripheral Blood Mononuclear Cells of Subjects with Exposure to Nickel[J]. ENVIRONMENTAL HEALTH PERSPECTIVES,2012,120(2):198-203.
APA	Arita, A.,Niu, JP.,Qu, QS.,Zhao, NJ.,Ruan, Y.,...&Costa, M .(2012).Global Levels of Histone Modifications in Peripheral Blood Mononuclear Cells of Subjects with Exposure to Nickel.ENVIRONMENTAL HEALTH PERSPECTIVES,120(2),198-203.
MLA	Arita, A,et al."Global Levels of Histone Modifications in Peripheral Blood Mononuclear Cells of Subjects with Exposure to Nickel".ENVIRONMENTAL HEALTH PERSPECTIVES 120.2(2012):198-203.