A flavonoid compound from Chrysosplenium nudicaule inhibits growth and induces apoptosis of the human stomach cancer cell line SGC-7901

doi:10.3109/13880209.2015.1055634

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	A flavonoid compound from Chrysosplenium nudicaule inhibits growth and induces apoptosis of the human stomach cancer cell line SGC-7901
	Luo, YP; Yu, HJ; Yang, YS; Tian, WH; Dong, KZ; Shan, JY; Ma, XM; Ma, XM (reprint author), Lanzhou Univ, Sch Basic Med Sci, Dept Immunol, Lanzhou 730000, Peoples R China.
刊名	PHARMACEUTICAL BIOLOGY
	2016-07
卷号	54 期号:7 页码:1133-1139
关键词	Antitumor cell-cycle arrest Tibetan medicine
ISSN号	1388-0209
DOI	10.3109/13880209.2015.1055634
文献子类	Article
英文摘要	Context: Gastric cancer remains highly prevalent, but treatment options are limited. Natural products have proved to be a rich source of anticancer drugs. Chrysosplenium nudicaule Ledeb. (Saxifragaceae) is a perennial herb that grows in the highlands of China. It has been used as a traditional Chinese medicine to treat digestive diseases for hundreds of years. Recent studies revealed that this herb had anticancer activity, and the flavonoids were speculated to be the effective components. 6,7,3-Trimethoxy-3,5,4-trihydroxy flavone (TTF) and 5,4-dihydroxy-3,6,3trimethoxy-flavone-7-O--d-glucoside (DTFG) are flavonoid compounds isolated from Chrysosplenium nudicaule.Objective: This study examined the effect of TTF and DTFG on SGC-7901 human stomach cancer cell in vitro to determine the anticancer and induction of apoptosis properties of TTF.Materials and methods: The proliferation of cells treated with 32, 16, 8, 4, and 2g/mL of TTF or DTFG for 24, 48, and 72h was assessed by the MTT assay. After being treated with TTF, the apoptosis of SGC-7901 cells was assessed by acridine orange staining, ultrastructure, electrophoresis of DNA fragmentation, and flow cytometry.Results: Results indicated that TTF inhibited the growth of cancer cells with an IC50 value of 8.33g/mL after 72h incubation. However, DTFG showed no inhibitory effect on the growth of the cancer cell. Further studies on TTF also confirmed that it was able to induce apoptosis of SGC-7901 cells at a concentration as low as 4g/mL.Discussion and conclusion: The apoptotic effect of TTF makes it a promising candidate for future chemotherapeutic application in treating stomach cancer.
学科主题	Plant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy
出版地	ABINGDON
资助项目	国家自然科学基金项目
项目编号	National Natural Science Foundation of China [31360604]
语种	英语
WOS记录号	WOS:000379980500003
资助机构	NSFC
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179125]
专题	基础医学院_期刊论文
通讯作者	Ma, XM (reprint author), Lanzhou Univ, Sch Basic Med Sci, Dept Immunol, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Luo, YP,Yu, HJ,Yang, YS,et al. A flavonoid compound from Chrysosplenium nudicaule inhibits growth and induces apoptosis of the human stomach cancer cell line SGC-7901[J]. PHARMACEUTICAL BIOLOGY,2016,54(7):1133-1139.
APA	Luo, YP.,Yu, HJ.,Yang, YS.,Tian, WH.,Dong, KZ.,...&Ma, XM .(2016).A flavonoid compound from Chrysosplenium nudicaule inhibits growth and induces apoptosis of the human stomach cancer cell line SGC-7901.PHARMACEUTICAL BIOLOGY,54(7),1133-1139.
MLA	Luo, YP,et al."A flavonoid compound from Chrysosplenium nudicaule inhibits growth and induces apoptosis of the human stomach cancer cell line SGC-7901".PHARMACEUTICAL BIOLOGY 54.7(2016):1133-1139.