Mutation analysis underlying the downregulation of the thyroid hormone receptor beta 1 gene in the Chinese breast cancer population

doi:10.2147/OTT.S93418

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	Mutation analysis underlying the downregulation of the thyroid hormone receptor beta 1 gene in the Chinese breast cancer population
	Ling, YQ; Ling, XL; Fan, L; Wang, Y; Li, Q; Wang, Y (reprint author), Lanzhou Mil Command PLA, Lanzhou Gen Hosp, Dept Gastroenterol, 333 Binhe South Rd, Lanzhou 730050, Gansu, Peoples R China.
刊名	ONCOTARGETS AND THERAPY
	2015
卷号	8 页码:2967-2972
关键词	breast cancer mutation thyroid hormone receptor beta 1 biomarker
ISSN号	1178-6930
DOI	10.2147/OTT.S93418
文献子类	Article
英文摘要	Purpose: There are a growing number of reports suggesting that the aberrant expression and mutation of the thyroid hormone receptor beta 1 (TR beta 1) gene is associated with the development of human neoplasms. However, its exact role in the pathogenesis of breast cancer remains elusive. In the present study, we analyzed the mRNA expression and mutations of the TR beta 1 gene in the Chinese breast cancer population. Methods: The expression of TR beta 1 mRNA was examined by real-time quantitative reverse transcription polymerase chain reaction, and mutations in the TR beta 1 gene in the hotspot region that spans exons 7-10 were analyzed by polymerase chain reaction single-strand conformation polymorphism and automated DNA sequencing. Results: TR beta 1 mRNA expression was significantly reduced in all 105 breast cancer specimens examined. A total of 20 samples showed truncating mutations within the exons 7-10 of the TR beta 1 gene, where eight cases harbored a frame shift mutation (five cases of c.850insA in exon 7 and three cases c.1028delA in exon 8), whereas missense mutations were observed in 12 breast cancer cases. The 20 cases with mutation in the TR beta 1 gene showed a reduction in TR beta 1 mRNA expression compared with that observed in matched normal tissues. The mutation was also correlated with menopausal stage and estrogen receptor status. Conclusion: The findings of the present study suggest that the aberrant expression and mutations of the TR beta 1 gene are associated with the development of breast cancer and that the-mutations in the TR beta 1 gene partly serve as the underlying mechanism for TR beta 1 inactivation in the Chinese breast cancer population.
学科主题	Biotechnology & Applied Microbiology ; Oncology
出版地	ALBANY
资助项目	甘肃省国际科技合作计划 ; 甘肃省科技支撑计划
项目编号	Pedestal - Project of Gansu Provincial Science [090NKCA126] ; Medical Research Fund of PLA [CLZ 11JB05] ; International Scientific and Technological Cooperation Project in Gansu Province [1011WCGA166]
语种	英语
WOS记录号	WOS:000363161600001
资助机构	GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/179000]
专题	基础医学院_期刊论文
通讯作者	Wang, Y (reprint author), Lanzhou Mil Command PLA, Lanzhou Gen Hosp, Dept Gastroenterol, 333 Binhe South Rd, Lanzhou 730050, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Ling, YQ,Ling, XL,Fan, L,et al. Mutation analysis underlying the downregulation of the thyroid hormone receptor beta 1 gene in the Chinese breast cancer population[J]. ONCOTARGETS AND THERAPY,2015,8:2967-2972.
APA	Ling, YQ,Ling, XL,Fan, L,Wang, Y,Li, Q,&Wang, Y .(2015).Mutation analysis underlying the downregulation of the thyroid hormone receptor beta 1 gene in the Chinese breast cancer population.ONCOTARGETS AND THERAPY,8,2967-2972.
MLA	Ling, YQ,et al."Mutation analysis underlying the downregulation of the thyroid hormone receptor beta 1 gene in the Chinese breast cancer population".ONCOTARGETS AND THERAPY 8(2015):2967-2972.