Modulation of microRNAs by ionizing radiation in human gastric cancer

doi:10.3892/or.2014.3246

CORC > 兰州大学 > 兰州大学 > 基础医学院 > 期刊论文

	Modulation of microRNAs by ionizing radiation in human gastric cancer
	He, JP; Hua, JR; Ding, N; Xu, S; Sun, R; Zhou, GM; Xie, XD; Wang, JF; Wang, JF (reprint author), Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 73000, Peoples R China.
刊名	ONCOLOGY REPORTS
	2014-08-05
卷号	32 期号:2 页码:787-793
关键词	microRNA ionizing radiation gastric cancer miR-300 miR-642
ISSN号	1021-335X
DOI	10.3892/or.2014.3246
文献子类	Article
英文摘要	Gastric cancer is one of the most common cancers in China. Although surgery is the primary therapeutic method, radiotherapy has become an integral part, particularly in the early and intermediate stages of gastric cancer. microRNAs (miRNAs) are involved in the regulation of diverse cellular processes in response to intrinsic and extrinsic stress. A change in miRNA expression profile has been identified in various types of tumor cells in response to radiation; however, there is no relevant information concerning gastric cancer. In the present study, we investigated the miRNA profiles of two clinical gastric cancer samples exposed to X-rays using miRNA microarray. We found that 16 miRNAs were downregulated and 2 miRNAs were upregulated significantly in both irradiated samples when compared with the unirradiated samples. Decreases in the levels of miR-300 and miR-642 expression were confirmed by qRT-PCR in more clinical samples and in cultured cell lines. We predicted the targets of the two miRNAs with Target Scan and classified all the candidate targets with Gene Ontology, which indicated that both miR-300 and miR-642 potentially regulate cellular radiation response by modulating apoptosis, cell cycle regulation and DNA damage and repair pathway-related genes. Cell cycle assay and immunofluorescence assay demonstrated that miR-300 regulates radiation-induced G2 cell cycle arrest and DNA damage repair. In conclusion, our findings indicate that ionizing radiation modulates the miRNA expression profile, and the changes in several specific miRNAs such as miR-300 have the potential to be used in the treatment, diagnosis and prognosis of gastric cancer.
学科主题	Oncology
出版地	ATHENS
资助项目	国家自然科学基金项目
项目编号	National Natural Science Foundation of China [31270895, 81272454, U1232125]
语种	英语
WOS记录号	WOS:000338908700043
资助机构	NSFC
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121706]
专题	基础医学院_期刊论文
通讯作者	Wang, JF (reprint author), Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 73000, Peoples R China.
推荐引用方式 GB/T 7714	He, JP,Hua, JR,Ding, N,et al. Modulation of microRNAs by ionizing radiation in human gastric cancer[J]. ONCOLOGY REPORTS,2014,32(2):787-793.
APA	He, JP.,Hua, JR.,Ding, N.,Xu, S.,Sun, R.,...&Wang, JF .(2014).Modulation of microRNAs by ionizing radiation in human gastric cancer.ONCOLOGY REPORTS,32(2),787-793.
MLA	He, JP,et al."Modulation of microRNAs by ionizing radiation in human gastric cancer".ONCOLOGY REPORTS 32.2(2014):787-793.