Therapeutic effect and mechanism of proanthocyanidins from grape seeds in rats with TNBS-induced ulcerative colitis

doi:10.1139/Y08-089

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	Therapeutic effect and mechanism of proanthocyanidins from grape seeds in rats with TNBS-induced ulcerative colitis
	Li, XL; Cai, YQ; Qin, H; Wu, YJ; Wu, YJ (reprint author), Lanzhou Univ, Sch Basic Med Sci, Dept Pharmacol, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China.
刊名	CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
	2008-12
卷号	86 期号:12 页码:841-849
关键词	proanthocyanidins from grape seeds ulcerative colitis trinitrobenzene sulfonic acid antiinflammatory antioxidation interleukin
ISSN号	0008-4212
DOI	10.1139/Y08-089
文献子类	Article
英文摘要	The aim of the study was to investigate the therapeutic effect and mechanism of proanthocyanidins from grape seeds (GSPE) in the treatment of ulcerative colitis (UC). Rats were intragastrically administered different doses of GSPE (100, 200, and 400 mg/kg) per day for 7 days after UC was twice-induced by intracolonic injection of 2,4,6-trinitrobenzene sulfonic acid (TNBS) dissolved in 50% ethanol. Sulfasalazine (SASP) at 200 mg/kg was used as a positive control drug. Macroscopic and microscopic damage scores and changes in weight/length ratio (mg/mm) of colon segments were analyzed. The levels of malonyldialdehyde (MDA), interleukin (IL)-1b, IL-2, IL-4, and myeloperoxidase (MPO) activity in the colon tissues and MPO activity in the serum were all measured by biochemical methods or double antibody sandwich ELISA methods. Compared with the TNBS control group, GSPE treatment facilitated recovery of pathologic changes in the colon after insult with TNBS, as demonstrated by increased body weight (p < 0.01) and decreased colonic weight/length ratio (p < 0.01); GSPE also notably reduced the colonic macroscopic and microscopic damage scores (p < 0.01). The MPO activity in colon tissues and serum of rats treated with GSPE was significantly lower than that in the TNBS control group. The MDA and IL-1 beta levels of colon tissues were also decreased in GSPE groups. The intestinal antiinflammatory effect of GSPE was accompanied by a significant improvement of IL-2 and IL-4 levels in the colon tissues of rats in the high-dose GSPE group (p < 0.05). Compared with the SASP group, GSPE groups had no significant difference in the therapeutic effect (p > 0.05). GSPE exerts a beneficial antiinflammatory effect in the acute phase of TNBS-induced colitis in rats by downregulating some of the mediators involved in the intestinal inflammatory response, inhibiting inflammatory cell infiltration and antioxidation damage, promoting damaged tissue repair to improve colonic oxidative stress, decreasing production of proinflammatory cytokines IL-1b, and increasing production of antiinflammatory cytokines IL-2 and IL-4.
学科主题	Pharmacology & Pharmacy ; Physiology
出版地	OTTAWA
资助项目	国家自然科学基金项目 ; 甘肃省自然科学基金计划
项目编号	Natural Science Foundation of Gansu Province [3ZS061-A25-083] ; National Natural Science Foundation of China [30672490]
语种	英语
WOS记录号	WOS:000262400800004
资助机构	NSFC ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121396]
专题	基础医学院_期刊论文
通讯作者	Wu, YJ (reprint author), Lanzhou Univ, Sch Basic Med Sci, Dept Pharmacol, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Li, XL,Cai, YQ,Qin, H,et al. Therapeutic effect and mechanism of proanthocyanidins from grape seeds in rats with TNBS-induced ulcerative colitis[J]. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY,2008,86(12):841-849.
APA	Li, XL,Cai, YQ,Qin, H,Wu, YJ,&Wu, YJ .(2008).Therapeutic effect and mechanism of proanthocyanidins from grape seeds in rats with TNBS-induced ulcerative colitis.CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY,86(12),841-849.
MLA	Li, XL,et al."Therapeutic effect and mechanism of proanthocyanidins from grape seeds in rats with TNBS-induced ulcerative colitis".CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY 86.12(2008):841-849.