Investigation on the interaction between endocrine disruptor triphenyltin with human serum albumin

doi:10.1016/j.saa.2013.10.010

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	Investigation on the interaction between endocrine disruptor triphenyltin with human serum albumin
	Geng, Bing 1; Liu, Xue 1; Tian, Yunlong 1; Ye, Jing 1; Li, Hongna 1; Wu, Jinbao 2
刊名	SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
	2014
卷号	120 页码:512-516
关键词	Human serum albumin (HSA) Triphenyltin (TPT) Fluorescence spectra Toxicity
ISSN号	1386-1425
DOI	10.1016/j.saa.2013.10.010
通讯作者	Geng, Bing
英文摘要	The interaction between triphenyltin (TPT) and human serum albumin (HSA) in physiological buffer (pH = 7.4) was investigated by the fluorescence quenching technique. The results of fluorescence titration revealed that TPT could strongly quench the intrinsic fluorescence of HSA through a static quenching procedure. The apparent binding constants K and number of binding sites n of TPT with HSA were 2.51 x 10(3) and 0.96 at 298 K which were obtained by the fluorescence quenching method. The thermodynamic parameters enthalpy change (Delta H), entropy change (Delta S) were positive, which indicated that the interaction of TPT with HSA was driven mainly by hydrophobic forces. The process of binding was a spontaneous process in which Gibbs free energy change was negative. The distance r between donor (HSA) and acceptor (TPT) was calculated to be 3.13 nm based on Forster's non-radiative energy transfer theory. The results of synchronous fluorescence, three-dimensional fluorescence and circular dichroism (CD) spectra showed that the triphenyltin induced conformational changes of HSA. (C) 2013 Elsevier B.V. All rights reserved.
学科主题	Spectroscopy ; SPECTROSCOPY
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000331342500066
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/19809]
专题	农业环境与可持续发展研究所_环境修复研究室
作者单位	1.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing 100081, Peoples R China 2.Shenyang Univ Chem Technol, Shenyang 110142, Peoples R China
推荐引用方式 GB/T 7714	Geng, Bing,Liu, Xue,Tian, Yunlong,et al. Investigation on the interaction between endocrine disruptor triphenyltin with human serum albumin[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2014,120:512-516.
APA	Geng, Bing,Liu, Xue,Tian, Yunlong,Ye, Jing,Li, Hongna,&Wu, Jinbao.(2014).Investigation on the interaction between endocrine disruptor triphenyltin with human serum albumin.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,120,512-516.
MLA	Geng, Bing,et al."Investigation on the interaction between endocrine disruptor triphenyltin with human serum albumin".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 120(2014):512-516.