蔗渣发酵菌剂培养基的筛选及发酵条件的优化

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	蔗渣发酵菌剂培养基的筛选及发酵条件的优化
	叶婧; 刘雪; 张丽; 于江; 朱昌雄
刊名	生物技术通报
	2009
期号	11 页码:168-173
关键词	蔗渣发酵菌剂培养基优化发酵条件优化
ISSN号	1002-5464
其他题名	Screening of Fermentation Medium and Optimizing Fermentation Conditions of the Bagasse Microorganism Inoculants
英文摘要	采用单因素和正交试验研究了蔗渣高效发酵菌剂(芽孢杆菌B-A、曲霉菌F-A、链霉菌A-B)的摇瓶发酵最佳工艺条件.结果表明:芽孢杆菌B-A的最佳培养基配方:牛肉膏0.3%、蛋白胨1%、葡萄糖1%、NaCl 0.5%、可溶性淀粉0.5%、3.08%浓度的MnSO_4溶液0.1;最适发酵条件为pH7、装液量100 ml(250 ml三角瓶)、36℃培养27 h.曲霉F-A的最佳培养基配方:葡萄糖3%、豆饼粉3%、蛋白胨1.2%、酵母膏0.3%、K_2HPO_4 0.05%、KH_2PO_4 0.05%、CaCl_2 0.08%、MgSO_4 0.04%、MnSO_4 0.04%、ZnSO_4 0.02%;最适发酵条件为pH6、装液量50 ml、30℃培养3 d.链霉菌A-B的最佳培养基配方:可溶性淀粉4.5%、蔗糖1%、豆饼粉3%、NaNO_3 0.2%、ZnSO_4 0.01%、KH_2PO_4 0.001%;最适发酵条件为pH7、装液量50 ml、30℃培养3 d.; The optimal conditions for flask fermentation of the strains Bacillus sp. B-A,Aspergillus sp. F-A and Streptomyces sp. A-B) were studied by the single-factor and orthogonal test. The results showed that the optimal medium composition ( % ) for Bacillus sp. B-A was beef extract 0.3,peptone 1 ,glucose 1, NaCl 0.5, soluble starch 0. 5 ,3. 08% MnS0_4 0. 1; the optimum conditions were initial pH of 7,media volume 100ml,shaking at 36℃ for 27 hours. The optimal medium composition (%) of Aspergillus sp. F-A was glucose 3 ,soybean powder 3 .peptone 1. 2 ,yeast extract 0. 3 , K_2HPO_40. 05 ,KH_2PO_4 0. 05 ,CaCl_2 0. 08 、MgSO_40. 04,MnSO_4 0. 04 ,ZnSO_4 0.02. The optimum conditions were initial pH of 6, media volume 50ml, shaking at 30℃ for 3 days. The optimal medium composition ( % ) of Streptomyces sp. A-B: soluble starch 4. 5 , soybean cake powder 3 , sucrose 1, NaNO_3 0. 2, ZnSO_4 0. 01 , KH_2 PO_4 0. 001. The optimum conditions were initial pH of 7 , media volume 50 ml, shaking at 30℃ for 3 days.
学科主题	化学工业
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/17295]
专题	农业环境与可持续发展研究所_环境修复研究室
作者单位	中国农业科学院农业环境与可持续发展研究所, 北京, 100081
推荐引用方式 GB/T 7714	叶婧,刘雪,张丽,等. 蔗渣发酵菌剂培养基的筛选及发酵条件的优化[J]. 生物技术通报,2009(11):168-173.
APA	叶婧,刘雪,张丽,于江,&朱昌雄.(2009).蔗渣发酵菌剂培养基的筛选及发酵条件的优化.生物技术通报(11),168-173.
MLA	叶婧,et al."蔗渣发酵菌剂培养基的筛选及发酵条件的优化".生物技术通报 .11(2009):168-173.