反硝化细菌法结合痕量气体分析仪/同位素比质谱仪分析水体硝酸盐氮同位素组成

CORC > 中国农业科学院 > 农业环境与可持续发展研究所 > 旱作节水研究室

	反硝化细菌法结合痕量气体分析仪/同位素比质谱仪分析水体硝酸盐氮同位素组成
	徐春英; 李玉中; 郝卫平; 李巧珍; 董一威; 房福力; 郭智成
刊名	分析化学
	2012
卷号	40 期号:9 页码:1360-1365
关键词	反硝化细菌水体硝酸盐氮同位素痕量气体同位素比质谱
ISSN号	0253-3820
其他题名	Analysis of Nitrogen Isotopic Composition of Nitrate in Water by Denitrifier Method and Trace-Gas/Isotope Ratio Mass Spectrometry
英文摘要	建立了反硝化细菌法结合痕量气体分析仪(TraceGas)/同位素比质谱仪分析水体硝酸盐氮同位素组成的方法。对反硝化细菌生长、培养条件和方法的精密度及稳定性进行了分析,并利用标准样品USGS34研究了样品反硝化孵育时间、TraceGas捕集N_2O气体时间对δ~(15)N测定的影响。结果表明:恰当的氧气量才能培养出有效的反硝化细菌;本方法精密度及稳定性较好,同一制备时间内硝酸盐δ~(15)N的SD在0.09‰~0.14‰之间,6个月内SD为0.12‰;样品反硝化孵育3~24 h可以得到稳定的δ~(15)N;TraceGas捕集时间为500 s时得到的δ~(15)N校正值与真实值最接近。应用本方法对养殖场污水和灌溉井水的硝酸盐δ~(15)N进行了测定。; A method based on denitrifying with TraceGas and Isotope Ratio Mass Spectrometry(IRMS) has been developed for the determination of nitrogen isotopic composition of nitrate in water samples. Bacterial growth, culture conditions and the precision, stability of method were investigated. The sample incubation time and trapping time by TraceGas affecting δ~(15)N were examined using standard reference material USGS34. It is very important to obtain bacterial growth success by controlling the volume of oxygen in the culture. The method has good precision and stability with the standard deviations (SD) from 0.09‰ to 0.14‰ in the same sample preparation time and 0.12‰ in 6 months. The values of δ~(15)N were almost the same from 3 h to 24 h for the sample incubation time course. 500 s was the best trapping time by TraceGas for the smallest difference between corrected values and real values. The δ~(15) N values of nitrate in the livestock wastewater and irrigating water were measured by this method.
学科主题	化学 ; 环境质量评价与环境监测
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/17058]
专题	农业环境与可持续发展研究所_旱作节水研究室
作者单位	中国农业科学院农业环境与可持续发展研究所, 农业部旱作节水农业重点开放实验室, 北京, 100081
推荐引用方式 GB/T 7714	徐春英,李玉中,郝卫平,等. 反硝化细菌法结合痕量气体分析仪/同位素比质谱仪分析水体硝酸盐氮同位素组成[J]. 分析化学,2012,40(9):1360-1365.
APA	徐春英.,李玉中.,郝卫平.,李巧珍.,董一威.,...&郭智成.(2012).反硝化细菌法结合痕量气体分析仪/同位素比质谱仪分析水体硝酸盐氮同位素组成.分析化学,40(9),1360-1365.
MLA	徐春英,et al."反硝化细菌法结合痕量气体分析仪/同位素比质谱仪分析水体硝酸盐氮同位素组成".分析化学 40.9(2012):1360-1365.