RNA-Seq-Based Transcriptome Profiling of Early Nitrogen Deficiency Response in Cucumber Seedlings Provides New Insight into the Putative Nitrogen Regulatory Network

doi:10.1093/pcp/pcu172

	RNA-Seq-Based Transcriptome Profiling of Early Nitrogen Deficiency Response in Cucumber Seedlings Provides New Insight into the Putative Nitrogen Regulatory Network
	Zhao, Wenchao 1,2; Yang, Xueyong 1; Yu, Hongjun 1; Jiang, Weijie 1; Sun, Na 1; Liu, Xiaoran 1; Liu, Xiaolin 1; Zhang, Xiaomeng 1; Wang, Yan 1; Gu, Xingfang 1
刊名	PLANT AND CELL PHYSIOLOGY
	2015
卷号	56 期号:3 页码:455-467
关键词	Ascorbic acid Cell wall Cucumber Nitrogen deficiency Regulatory network RNA-Seq
ISSN号	0032-0781
DOI	10.1093/pcp/pcu172
通讯作者	Zhao, Wenchao
英文摘要	Nitrogen (N) is both an important macronutrient and a signal for plant growth and development. However, the early regulatory mechanism of plants in response to N starvation is not well understood, especially in cucumber, an economically important crop that normally consumes excessive N during production. In this study, the early time-course transcriptome response of cucumber leaves under N deficiency was monitored using RNA sequencing (RNA-Seq). More than 23,000 transcripts were examined in cucumber leaves, of which 364 genes were differentially expressed in response to N deficiency. Based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, gene ontology (GO) and protein-protein interaction analysis, 64 signaling-related N-deficiency-responsive genes were identified. Furthermore, the potential regulatory mechanisms of anthocyanin accumulation, Chl decline and cell wall remodeling were assessed at the transcription level. Increased ascorbic acid synthesis was identified in cucumber seedlings and fruit under N-deficient conditions, and a new corresponding regulatory hypothesis has been proposed. A data cross-comparison between model plants and cucumber was made, and some common and specific N-deficient response mechanisms were found in the present study. Our study provides novel insights into the responses of cucumber to nitrogen starvation at the global transcriptome level, which are expected to be highly useful for dissecting the N response pathways in this major vegetable and for improving N fertilization practices.
学科主题	Plant Sciences ; Cell Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000352494100007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100918]
专题	蔬菜花卉研究所_葫芦科研究室
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Minist Agr, Key Lab Hort Crops Genet Improvement, Beijing 100081, Peoples R China 2.Beijing Univ Agr, Plant Sci & Technol Coll, Beijing Key Lab Agr Applicat & New Technol, Beijing 102206, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Wenchao,Yang, Xueyong,Yu, Hongjun,et al. RNA-Seq-Based Transcriptome Profiling of Early Nitrogen Deficiency Response in Cucumber Seedlings Provides New Insight into the Putative Nitrogen Regulatory Network[J]. PLANT AND CELL PHYSIOLOGY,2015,56(3):455-467.
APA	Zhao, Wenchao.,Yang, Xueyong.,Yu, Hongjun.,Jiang, Weijie.,Sun, Na.,...&Gu, Xingfang.(2015).RNA-Seq-Based Transcriptome Profiling of Early Nitrogen Deficiency Response in Cucumber Seedlings Provides New Insight into the Putative Nitrogen Regulatory Network.PLANT AND CELL PHYSIOLOGY,56(3),455-467.
MLA	Zhao, Wenchao,et al."RNA-Seq-Based Transcriptome Profiling of Early Nitrogen Deficiency Response in Cucumber Seedlings Provides New Insight into the Putative Nitrogen Regulatory Network".PLANT AND CELL PHYSIOLOGY 56.3(2015):455-467.