MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF UNUSUAL X-TYPE HMW GLUTENIN SUBUNITS FROM 1S(L) GENOME OF Aegilops longissima

doi:10.2298/GENSR1501185Z

	MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF UNUSUAL X-TYPE HMW GLUTENIN SUBUNITS FROM 1S(L) GENOME OF Aegilops longissima
	Zhu, Gengrui 1; Wang, Shunli 1,2; Zhen, Shoumin 1; Shen, Xixi 1; Prodanovic, Slaven 3; Yan, Yueming 1,4
刊名	GENETIKA-BELGRADE
	2015
卷号	47 期号:1 页码:185-203
关键词	Ae. longissima HMW-GS molecular clone phylogenetics
ISSN号	0534-0012
DOI	10.2298/GENSR1501185Z
通讯作者	Zhu, Gengrui
英文摘要	Wheat related diploid species Ae. longissima (2n=2x=14, (SSl)-S-l) has extensive storage protein variations that may provide useful gene resources for wheat quality improvement. In this work, five novel 1S(l)-encoded x-type high molecular glutenin subunits (HMW-GS) were identified and designated as 1S(l)x-123, 1S(l)x-129, 1S(l)x1-136, 1S(l)x2-136 and 1S(l)x2.2, respectively. Their complete open reading frames (ORFs) were cloned and sequenced by AS-PCR, which contained 2874 bp (956 aa) for 1S(l)x-123, 2946 bp (979 aa) for 1S(l)x-129, 2901 bp (965 aa) for 1S(l)x1-136, 2982bp (991 aa) for 1S(l)x2-136 and 2928 bp (974 aa) for 1S(l)x2.2. Molecular characterization demonstrated that five unusual subunits had greater repetitive domains resulted from a larger fragment insertion (74-113 aa). Particularly, 1S(l)x-129 had an extra cysteine residue at the position 109 due to a TAT -> TGT dot mutation, which may improve the formation of superior gluten macropolymer. Our results suggest that these unusual HMW-GS could be served as potential superior gene resources for improving wheat gluten quality. Phylogenetic analysis revealed that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with those of B genome.
学科主题	Agronomy ; Genetics & Heredity
语种	英语
出版者	SERBIAN GENETICS SOC
WOS记录号	WOS:000355248900017
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100933]
专题	蔬菜花卉研究所_花卉研究室
作者单位	1.Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100193, Peoples R China 3.Univ Belgrade, Fac Agr, Belgrade 11080, Serbia 4.Hubei Collaborat Innovat Ctr Grain Ind, Jingzhou 434025, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Gengrui,Wang, Shunli,Zhen, Shoumin,et al. MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF UNUSUAL X-TYPE HMW GLUTENIN SUBUNITS FROM 1S(L) GENOME OF Aegilops longissima[J]. GENETIKA-BELGRADE,2015,47(1):185-203.
APA	Zhu, Gengrui,Wang, Shunli,Zhen, Shoumin,Shen, Xixi,Prodanovic, Slaven,&Yan, Yueming.(2015).MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF UNUSUAL X-TYPE HMW GLUTENIN SUBUNITS FROM 1S(L) GENOME OF Aegilops longissima.GENETIKA-BELGRADE,47(1),185-203.
MLA	Zhu, Gengrui,et al."MOLECULAR CHARACTERIZATION AND PHYLOGENETIC ANALYSIS OF UNUSUAL X-TYPE HMW GLUTENIN SUBUNITS FROM 1S(L) GENOME OF Aegilops longissima".GENETIKA-BELGRADE 47.1(2015):185-203.