Genetic association of ETHYLENE-INSENSITIVE3-like sequence with the sex-determining M locus in cucumber (Cucumis sativus L.)

doi:10.1007/s00122-008-0832-1

	Genetic association of ETHYLENE-INSENSITIVE3-like sequence with the sex-determining M locus in cucumber (Cucumis sativus L.)
	Liu, Shiqiang 1,7; Xu, Liang 2,6; Jia, Zhiqi 1; Xu, Yong 3; Yang, Qing 7; Fei, Zhangjun 4,5; Lu, Xiangyang 2,6; Chen, Huiming 2,6; Huang, Sanwen 1
刊名	THEORETICAL AND APPLIED GENETICS
	2008
卷号	117 期号:6 页码:927-933
ISSN号	0040-5752
DOI	10.1007/s00122-008-0832-1
通讯作者	Liu, Shiqiang
英文摘要	Cucumber (Cucumis sativus L.) has served as the model system for sex expression in flowering plants and its sex type is predominantly controlled by two genetic loci, F and M. Ethylene is the major plant hormone that regulates sex expression in cucumber. The current model predicts that ethylene serves as both a promoter of femaleness via the F locus and an inhibitor of the male sex via the M locus. In support of this model, genetic, genomic, and transcript analyses indicate that the F gene encodes a key enzyme in ethylene biosynthesis. In this study, we discovered that the M locus co-segregates with an ETHYLENE-INSENSITIVE3 (EIN3)-like genomic sequence in an F(2) population of 96 individuals. This genetic association agrees with the prediction that the M locus is involved in ethylene signaling, thus providing another line of evidence in favor of the model. In addition, we generated an amplified fragment length polymorphism (AFLP((R))) map of the M locus, which was delimited into a genetic interval of 2.5 cM. The genetic association and the local map will assist the molecular isolation of the M gene using the combination of positional cloning and candidate gene approach.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000259276700009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101228]
专题	蔬菜花卉研究所_生物技术研究室
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Hunan Acad Agr Sci, Hunan Vegetable Res Inst, Changsha 410125, Peoples R China 3.Beijing Vegetable Res Ctr, Beijing 100089, Peoples R China 4.Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA 5.Cornell Univ, USDA Plant Soil & Nutr Lab, Ithaca, NY 14853 USA 6.Hunan Agr Univ, Lab Biochem & Fermentat, Changsha 410128, Hunan, Peoples R China 7.Nanjing Agr Univ, Coll Life Sci, Dept Biochem & Mol Biol, Nanjing 210095, Peoples R China
推荐引用方式 GB/T 7714	Liu, Shiqiang,Xu, Liang,Jia, Zhiqi,et al. Genetic association of ETHYLENE-INSENSITIVE3-like sequence with the sex-determining M locus in cucumber (Cucumis sativus L.)[J]. THEORETICAL AND APPLIED GENETICS,2008,117(6):927-933.
APA	Liu, Shiqiang.,Xu, Liang.,Jia, Zhiqi.,Xu, Yong.,Yang, Qing.,...&Huang, Sanwen.(2008).Genetic association of ETHYLENE-INSENSITIVE3-like sequence with the sex-determining M locus in cucumber (Cucumis sativus L.).THEORETICAL AND APPLIED GENETICS,117(6),927-933.
MLA	Liu, Shiqiang,et al."Genetic association of ETHYLENE-INSENSITIVE3-like sequence with the sex-determining M locus in cucumber (Cucumis sativus L.)".THEORETICAL AND APPLIED GENETICS 117.6(2008):927-933.