Integration of High-Resolution Physical and Genetic Map Reveals Differential Recombination Frequency between Chromosomes and the Genome Assembling Quality in Cucumber

doi:10.1371/journal.pone.0062676

	Integration of High-Resolution Physical and Genetic Map Reveals Differential Recombination Frequency between Chromosomes and the Genome Assembling Quality in Cucumber
	Lou, Qunfeng 1; He, Yuhua 1; Cheng, Chunyan 1; Zhang, Zhonghua 2; Li, Ji 1; Huang, Sanwen 2; Chen, Jinfeng 1
刊名	PLOS ONE
	2013
卷号	8 期号:5 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0062676
通讯作者	Lou, Qunfeng
英文摘要	Cucumber is an important model crop and the first species sequenced in Cucurbitaceae family. Compared to the fast increasing genetic and genomics resources, the molecular cytogenetic researches in cucumber are still very limited, which results in directly the shortage of relation between plenty of physical sequences or genetic data and chromosome structure. We mapped twenty-three fosmids anchored by SSR markers from LG-3, the longest linkage group, and LG-4, the shortest linkage group on pachytene chromosomes 3 and 4, using uorescence in situ hybridization (FISH). Integrated molecular cytogenetic maps of chromosomes 3 and 4 were constructed. Except for three SSR markers located on heterochromatin region, the cytological order of markers was concordant with those on the linkage maps. Distinct structural differences between chromosomes 3 and 4 were revealed by the high resolution pachytene chromosomes. The extreme difference of genetic length between LG-3 and LG-4 was mainly attributed to the difference of overall recombination frequency. The significant differentiation of heterochromatin contents in chromosomes 3 and 4 might have a direct correlation with recombination frequency. Meanwhile, the uneven distribution of recombination frequency along chromosome 4 was observed, and recombination frequency of the long arm was nearly 3.5 times higher than that of the short arm. The severe suppression of recombination was exhibited in centromeric and heterochromatin domains of chromosome 4. Whereas a close correlation between the gene density and recombination frequency was observed in chromosome 4, no significant correlation was observed between them along chromosome 3. The comparison between cytogenetic and sequence maps revealed a large gap on the pericentromeric heterochromatin region of sequence map of chromosome 4. These results showed that integrated molecular cytogenetic maps can provide important information for the study of genetic and genomics in cucumber.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000321390200033
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101053]
专题	蔬菜花卉研究所_生物技术研究室
作者单位	1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Lou, Qunfeng,He, Yuhua,Cheng, Chunyan,et al. Integration of High-Resolution Physical and Genetic Map Reveals Differential Recombination Frequency between Chromosomes and the Genome Assembling Quality in Cucumber[J]. PLOS ONE,2013,8(5):-.
APA	Lou, Qunfeng.,He, Yuhua.,Cheng, Chunyan.,Zhang, Zhonghua.,Li, Ji.,...&Chen, Jinfeng.(2013).Integration of High-Resolution Physical and Genetic Map Reveals Differential Recombination Frequency between Chromosomes and the Genome Assembling Quality in Cucumber.PLOS ONE,8(5),-.
MLA	Lou, Qunfeng,et al."Integration of High-Resolution Physical and Genetic Map Reveals Differential Recombination Frequency between Chromosomes and the Genome Assembling Quality in Cucumber".PLOS ONE 8.5(2013):-.