A receptor-like protein RMC is involved in regulation of iron acquisition in rice

doi:10.1093/jxb/ert290

	A receptor-like protein RMC is involved in regulation of iron acquisition in rice
	Yang, An 1; Li, Yansu 2; Xu, Yunyun 3; Zhang, Wen-Hao 1,4
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2013
卷号	64 期号:16 页码:5009-5020
关键词	Fe concentration iron deficiency OsRMC rice (Oryza sativa) root system seed Fe concentration
ISSN号	0022-0957
DOI	10.1093/jxb/ert290
通讯作者	Yang, An
英文摘要	Iron (Fe) is one of the essential mineral elements for plant growth and development. Acquisition of Fe by plants is mediated by a complex network involving Fe mobilization, uptake by root cells, and transport within plants. Here, we evaluated the role of a previously clarified gene encoding a receptor-like protein from rice, OsRMC, in the regulation of Fe acquisition by comparing Fe concentration, biomass, and expression patterns of genes associated with Fe mobilization and transport in wild-type (WT) rice with those in OsRMC overexpression and RNA interference (RNAi) knockdown transgenic rice plants. Expression of OsRMC was upregulated in both shoots and roots upon exposure of WT to Fe-deficient medium. Expression levels of OsRMC were positively correlated with Fe concentration in rice plants under both Fe-sufficient and Fe-deficient conditions such that overexpression and RNAi lines had higher and lower Fe concentration in both roots and shoots than WT plants, respectively. Moreover, overexpression of OsRMC conferred greater accumulation of Fe in mature seeds under Fe-sufficient conditions. OsRMC may also play a role in regulation of Fe deficiency-induced changes in root growth, as evidenced by greater and smaller root systems of OsRMC overexpression lines and RNAi lines than WT under Fe-deficient conditions, respectively. Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants. These novel findings highlight an important role of OsRMC played in mediation of Fe acquisition and root growth in rice, particularly under Fe-deficient conditions.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000327510100014
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101027]
专题	蔬菜花卉研究所_栽培与采后技术研究室
作者单位	1.Chinese Acad Sci, State Key Lab Vegetat & Environm Change, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 3.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, Beijing 100093, Peoples R China 4.Chinese Acad Sci, Res Network Global Change Biol, Beijing Inst Life Sci, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Yang, An,Li, Yansu,Xu, Yunyun,et al. A receptor-like protein RMC is involved in regulation of iron acquisition in rice[J]. JOURNAL OF EXPERIMENTAL BOTANY,2013,64(16):5009-5020.
APA	Yang, An,Li, Yansu,Xu, Yunyun,&Zhang, Wen-Hao.(2013).A receptor-like protein RMC is involved in regulation of iron acquisition in rice.JOURNAL OF EXPERIMENTAL BOTANY,64(16),5009-5020.
MLA	Yang, An,et al."A receptor-like protein RMC is involved in regulation of iron acquisition in rice".JOURNAL OF EXPERIMENTAL BOTANY 64.16(2013):5009-5020.