Influence of cellulase gene knockdown by dsRNA interference on the development and reproduction of the pine wood nematode, Bursaphelenchus xylophilus

doi:10.1163/138855409X12469541205044

	Influence of cellulase gene knockdown by dsRNA interference on the development and reproduction of the pine wood nematode, Bursaphelenchus xylophilus
	Cheng, Xin-Yue 2; Dai, Su-Ming 1; Xiao, Luo 1; Xie, Bing-Yan 1
刊名	NEMATOLOGY
	2010
卷号	12 页码:225-233
关键词	beta-1,4-endoglucanases gene gene silencing plant-parasitic nematodes RNA interference
ISSN号	1388-5545
DOI	10.1163/138855409X12469541205044
通讯作者	Cheng, Xin-Yue
英文摘要	Cellulase genes are very important for plant-parasitic nematodes to move and feed within their host plants. The pine wood nematode, Bursaphelenchus xylophilus, causes destructive damage by killing pine trees. In this study, by employing dsRNA interference technology, knockdown of a cellulase gene (Bx-eng-1) of B. xylophilus was achieved and the biological effects of RNAi on the nematode were observed. The result showed that, after 24 h soaking, dsRNA of the Bx-eng-1 gene was effectively delivered into the nematode causing a post-transcriptional gene silencing and decrease in cellulase activity. Moreover, the number of F1 generation offspring was reduced significantly when the dsRNA-treated nematodes were cultured on fungal mats. We consider that cellulase is important to B. xylophilus because it not only hydrolyses cellulose of plant cell wall for its parasitism and penetration in host plants, but also influences its feeding, development and propagation on fungal mats.
学科主题	Zoology ; ZOOLOGY
语种	英语
出版者	BRILL ACADEMIC PUBLISHERS
WOS记录号	WOS:000277129500008
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101195]
专题	蔬菜花卉研究所_植物保护研究室
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Xin-Yue,Dai, Su-Ming,Xiao, Luo,et al. Influence of cellulase gene knockdown by dsRNA interference on the development and reproduction of the pine wood nematode, Bursaphelenchus xylophilus[J]. NEMATOLOGY,2010,12:225-233.
APA	Cheng, Xin-Yue,Dai, Su-Ming,Xiao, Luo,&Xie, Bing-Yan.(2010).Influence of cellulase gene knockdown by dsRNA interference on the development and reproduction of the pine wood nematode, Bursaphelenchus xylophilus.NEMATOLOGY,12,225-233.
MLA	Cheng, Xin-Yue,et al."Influence of cellulase gene knockdown by dsRNA interference on the development and reproduction of the pine wood nematode, Bursaphelenchus xylophilus".NEMATOLOGY 12(2010):225-233.