小蛾GluCI受体a亚基cDNA基因克隆和序列分析

	小蛾GluCI受体a亚基cDNA基因克隆和序列分析
	梁延坡 1; 吴青君 2; 刘长仲 1; 张友军 2; 王少丽 2; 徐宝云 2
刊名	植物保护
	2010
卷号	36 期号:4 页码:49-54
关键词	小菜蛾谷氨酸门控的氯离子通道受体反转录一多聚酶键式反应 RACE 序列分析
ISSN号	0529-1542
其他题名	Cloning and sequence analysis of cDNA of GluCl receptor alpha gene from the diamondback moth, Plutella xylostella
英文摘要	利用RT-PCR和RACE技术对小菜蛾[Plutella xylostella (L)]4龄幼虫谷氨酸门控的氯离子通道(GluCl)受体cDNA全长进行了克隆和序列分析. 通过设计简并性上、下游引物扩增出小菜蛾GluCl受体α亚基基因 192 bp的cDNA片段,根据得到的片段序列设计3'和5'特异性引物采用RACE技术进行3'和5'末端的克隆,获得了小菜蛾GluCl受体的cDNA的完整序列, GenBank登录号为GQ221939. 该基因全长2 144 bp,其开放阅读框 (ORF)1344 bp,编码447个氨基酸. 相似性分析表明:它与果蝇和赤拟谷盗的相似性均为73%,与埃及伊蚊的相似性为75%,与东亚飞蝗的相似性为79%;氨基酸分析后显示它具有GluClα亚基的典型特征,表明克隆的cDNA序列是小菜蛾GluCla亚基基因的序列; One full-length cDNA sequence of the GluCl receptor alpha gene of the fourth instars of the diamondback moth, Plutella xylostella (L.) was amplified, cloned, sequenced and analyzed by using RT-PCR and RACE method. One fragment of 192 bp cDNA of the GluCl receptor alpha gene was obtained by using degenerate forward and reverse primers. Then the 5'-and 3'-end specific primers were designed according to the fragment sequence for the 5'-and 3'-RACE.A 2 144 bp full-length cDNA of GluCl receptor was obtained. This gene was deposited in GenBank (accession no. GQ221939).It has an open reading frame of 1 344 nucleotides, encoding 447 amino acids residues. Similarity analysis showed that the cloned cDNA sequence had 73% identity with the published sequences of Drosophila melanogaster and Tribolium castaneum,75% and 79% identities with those of Aedes aegypti and Locusta migratoria. Typical features of GluCI subunits as in other members of the Cys-loop ligand-gated ion channel superfamily were found through protein analysis. The results indicated that the cDNA sequence was GluCla receptor gene
学科主题	昆虫学 ; 植物保护
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100655]
专题	蔬菜花卉研究所_植物保护研究室
作者单位	1.甘肃农业大学草业学院, 兰州, 730070 2.中国农业科学院蔬菜花卉研究所, 北京, 100081
推荐引用方式 GB/T 7714	梁延坡,吴青君,刘长仲,等. 小蛾GluCI受体a亚基cDNA基因克隆和序列分析[J]. 植物保护,2010,36(4):49-54.
APA	梁延坡,吴青君,刘长仲,张友军,王少丽,&徐宝云.(2010).小蛾GluCI受体a亚基cDNA基因克隆和序列分析.植物保护,36(4),49-54.
MLA	梁延坡,et al."小蛾GluCI受体a亚基cDNA基因克隆和序列分析".植物保护 36.4(2010):49-54.