Identification of candidate genes associated with male sterility in CMS7311 of heading Chinese cabbage (Brassica campestris L. ssp pekinensis)

doi:10.1007/s11738-013-1346-2

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	Identification of candidate genes associated with male sterility in CMS7311 of heading Chinese cabbage (Brassica campestris L. ssp pekinensis)
	Xu, Xiaoyong 1,2; Sun, Xilu 1,2; Zhang, Jing 1,2; Huang, Weiwei 1,3; Zhang, Lugang 1,2; Fang, Zhiyuan 2,4
刊名	ACTA PHYSIOLOGIAE PLANTARUM
	2013
卷号	35 期号:11 页码:3265-3270
关键词	cDNA-AFLP CMS Heading Chinese cabbage qRT-PCR Candidate gene
ISSN号	0137-5881
DOI	10.1007/s11738-013-1346-2
通讯作者	Xu, Xiaoyong
英文摘要	Polima (pol) cytoplasmic male sterility (CMS) is reported to be regulated by orf224, but the molecular mechanisms of CMS are less known. In previous research, CMS7311 of heading Chinese cabbage (Brassica campestris L.ssp. pekinensis) was successfully generated from a pol-like CMS, named 77A. To further understand the molecular mechanisms of this male sterility, cDNA-AFLP and qRT-PCR were used to identify genes differentially expressed during flower bud development between cytoplasmic male-sterile plant (Ms) and male-fertile plant (Mf) derived from the hybrid of CMS7311 and a restorer (Rf). Thirty-two transcripts of different fragments (TDFs) of over 80 bp in length were identified using cDNA-AFLP. Among them, ten TDFs were found expressing in Ms bud, and the others expressing in Mf bud. Gene ontology analysis revealed that these genes were involved in stress response, synthesis and metabolism of fatty acid, cell reconstruction, etc. Further, five genes closely related to male sterile were selected and their expression during the flower bud development of Ms and Mf line were investigated by qRT-PCR. Three genes, BcGRP17, BcMS2, and BcPME31, which were necessary for normal male organ development, showed significantly lower expressions in Ms bud; two genes, BcROPGEF8 and BcTNL3, were induced with significantly higher expression in Ms bud by unknown stress and involved in the formation of male sterility. Further work will be focused on cloning these genes and identifying their functions in the male-sterile process of heading Chinese cabbage CMS7311.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000325823300020
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101023]
专题	蔬菜花卉研究所_职能部门
作者单位	1.State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China 2.Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China 3.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China 4.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Xu, Xiaoyong,Sun, Xilu,Zhang, Jing,et al. Identification of candidate genes associated with male sterility in CMS7311 of heading Chinese cabbage (Brassica campestris L. ssp pekinensis)[J]. ACTA PHYSIOLOGIAE PLANTARUM,2013,35(11):3265-3270.
APA	Xu, Xiaoyong,Sun, Xilu,Zhang, Jing,Huang, Weiwei,Zhang, Lugang,&Fang, Zhiyuan.(2013).Identification of candidate genes associated with male sterility in CMS7311 of heading Chinese cabbage (Brassica campestris L. ssp pekinensis).ACTA PHYSIOLOGIAE PLANTARUM,35(11),3265-3270.
MLA	Xu, Xiaoyong,et al."Identification of candidate genes associated with male sterility in CMS7311 of heading Chinese cabbage (Brassica campestris L. ssp pekinensis)".ACTA PHYSIOLOGIAE PLANTARUM 35.11(2013):3265-3270.