Multiple copies of eukaryotic translation initiation factors in Brassica rapa facilitate redundancy, enabling diversification through variation in splicing and broad-spectrum virus resistance

doi:10.1111/tpj.12389

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	Multiple copies of eukaryotic translation initiation factors in Brassica rapa facilitate redundancy, enabling diversification through variation in splicing and broad-spectrum virus resistance
	Nellist, Charlotte F.1; Qian, Wei 2; Jenner, Carol E.1; Moore, Jonathan D.1; Zhang, Shujiang 2; Wang, Xiaowu 2; Briggs, William H.3; Barker, Guy C.1; Sun, Rifei 2; Walsh, John A.1
刊名	PLANT JOURNAL
	2014
卷号	77 期号:2 页码:261-268
关键词	Brassica rapa Turnip mosaic virus eIF(iso)4E broad-spectrum virus resistance mis-splicing Arabidopsis thaliana Chinese cabbage
ISSN号	0960-7412
DOI	10.1111/tpj.12389
通讯作者	Nellist, Charlotte F.
英文摘要	Recessive strain-specific resistance to a number of plant viruses in the Potyvirus genus has been found to be based on mutations in the eukaryotic translation initiation factor 4E (eIF4E) and its isoform, eIF(iso)4E. We identified three copies of eIF(iso)4E in a number of Brassica rapa lines. Here we report broad-spectrum resistance to the potyvirus Turnip mosaic virus (TuMV) due to a natural mechanism based on the mis-splicing of the eIF(iso)4E allele in some TuMV-resistant B.rapa var. pekinensis lines. Of the splice variants, the most common results in a stop codon in intron 1 and a much truncated, non-functional protein. The existence of multiple copies has enabled redundancy in the host plant's translational machinery, resulting in diversification and emergence of the resistance. Deployment of the resistance is complicated by the presence of multiple copies of the gene. Our data suggest that in the B.rapa subspecies trilocularis, TuMV appears to be able to use copies of eIF(iso)4E at two loci. Transformation of different copies of eIF(iso)4E from a resistant B.rapa line into an eIF(iso)4E knockout line of Arabidopsis thaliana proved misleading because it showed that, when expressed ectopically, TuMV could use multiple copies which was not the case in the resistant B.rapa line. The inability of TuMV to access multiple copies of eIF(iso)4E in B.rapa and the broad spectrum of the resistance suggest it may be durable.
学科主题	Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000337545400008
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101008]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Univ Warwick, Sch Life Sci, Wellesbourne CV35 9EF, Warwick, England 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 3.Syngenta Seeds, NL-1601 AA Enkhuizen, Netherlands
推荐引用方式 GB/T 7714	Nellist, Charlotte F.,Qian, Wei,Jenner, Carol E.,et al. Multiple copies of eukaryotic translation initiation factors in Brassica rapa facilitate redundancy, enabling diversification through variation in splicing and broad-spectrum virus resistance[J]. PLANT JOURNAL,2014,77(2):261-268.
APA	Nellist, Charlotte F..,Qian, Wei.,Jenner, Carol E..,Moore, Jonathan D..,Zhang, Shujiang.,...&Walsh, John A..(2014).Multiple copies of eukaryotic translation initiation factors in Brassica rapa facilitate redundancy, enabling diversification through variation in splicing and broad-spectrum virus resistance.PLANT JOURNAL,77(2),261-268.
MLA	Nellist, Charlotte F.,et al."Multiple copies of eukaryotic translation initiation factors in Brassica rapa facilitate redundancy, enabling diversification through variation in splicing and broad-spectrum virus resistance".PLANT JOURNAL 77.2(2014):261-268.