西花蓟马对多杀菌素的抗性生化机制硏究

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	西花蓟马对多杀菌素的抗性生化机制硏究
	侯文杰; 李飞; 吴青君; 徐宝云; 张友军
刊名	应用昆虫学报
	2013
卷号	50 期号:4 页码:1042-1048
关键词	西花蓟马多杀菌素抗性汰选交互抗性解毒酶系
ISSN号	2095-1353
其他题名	Biochemical mechanism of resistance to spinosad in Frankliniella occidentalis
英文摘要	在室内持续用多杀菌素对西花蓟马Frankliniella occidentalis (Pergande)进行抗性汰选,获得抗性倍数达到11 999倍的极高抗品系。该抗性品系对乙基多杀菌素和噻虫嗪具有显著的交互抗性,抗性倍数分别为53 718和84倍,对阿维菌素和毒死蜱的敏感性也有显著下降,交互抗性倍数分别为3.33和2.28倍,对虫螨腈无明显交互抗性。生化测定结果表明,抗性品系的羧酸酯酶、谷胱甘肽S-转移酶和多功能氧化酶的活力与敏感品系均无显著差异,推测抗性可能与靶标位点的敏感性降低有关。; Successive generations of the western flower thrips Frankliniella occidentalis (Pergande), were selected for resistance to spinosad. After 5 years we obtained a highly resistant strain, displaying 11 999-fold resistance to spinosad. The resistant strain also showed 53 718-and 84-fold cross-resistance, respectively, to spinetoram and thiamethoxam, low cross-resistance (3.33 and 2.28-fold, respectively) to abamectin and chlorpyrifos, but no observed cross-resistance to chlorfenapyr. Biochemical analysis showed that the activities of carboxylesterase, glutathione S-transferase and multifunctional oxidases in the resistant strain were not significantly different from those of the susceptible strain. These results indicate that metabolic detoxification does not play a role in the resistance of western flower thrips to spinosad, suggesting that spinosad may reduce sensitivities of the target sites.
学科主题	昆虫学
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100604]
专题	蔬菜花卉研究所_职能部门
作者单位	中国农业科学院蔬菜花卉研究所, 北京, 100081
推荐引用方式 GB/T 7714	侯文杰,李飞,吴青君,等. 西花蓟马对多杀菌素的抗性生化机制硏究[J]. 应用昆虫学报,2013,50(4):1042-1048.
APA	侯文杰,李飞,吴青君,徐宝云,&张友军.(2013).西花蓟马对多杀菌素的抗性生化机制硏究.应用昆虫学报,50(4),1042-1048.
MLA	侯文杰,et al."西花蓟马对多杀菌素的抗性生化机制硏究".应用昆虫学报 50.4(2013):1042-1048.