Fine mapping of a male sterility gene MS-cd1 in Brassica oleracea

doi:10.1007/s00122-011-1579-7

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Fine mapping of a male sterility gene MS-cd1 in Brassica oleracea
	Zhang, Xinmei 1,2; Wu, Jian 1; Zhang, Hui 1; Ma, Yuan 1,3; Guo, Aiguang 2; Wang, Xiaowu 1
刊名	THEORETICAL AND APPLIED GENETICS
	2011
卷号	123 期号:2 页码:231-238
ISSN号	0040-5752
DOI	10.1007/s00122-011-1579-7
通讯作者	Zhang, Xinmei
英文摘要	A dominant male sterility (DGMS) line 79-399-3, developed from a spontaneous mutation in Brassica oleracea var. capitata, has been widely used in production of hybrid cultivars in China. In this line, male sterility is controlled by a dominant gene Ms-cd1. In the present study, fine mapping of Ms-cd1 was conducted by screening a segregating population Ms79-07 with 2,028 individuals developed by four times backcrossing using a male sterile Brassica oleracea var. italica line harboring Ms-cd1 as donor and Brassica oleracea var. alboglabra as the recipient. Bulked segregation analysis (BSA) was performed for the BC(4) population Ms79-07 using 26,417 SRAP primer SRAPs and 1,300 SSRs regarding of male sterility and fertility. A high-resolution map surrounding Ms-cd1 was constructed with 14 SRAPs and one SSR. The SSR marker 8C0909 was closely linked to the MS-cd1 gene with a distance of 2.06 cM. Fourteen SRAPs closely linked to the target gene were identified; the closest ones on each side were 0.18 cM and 2.16 cM from Ms-cd1. Three of these SRAPs were successfully converted to dominant SCAR markers with a distance to the Ms-cd1 gene of 0.18, 0.39 and 4.23 cM, respectively. BLAST analysis with these SCAR marker sequences identified a collinear genomic region about 600 kb in scaffold 000010 on chromosomeA10 in B. rapa and on chromosome 5 in A. thaliana. These results provide additional information for map-based cloning of the Ms-cd1 gene and will be helpful for marker-assisted selection (MAS).
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture ; AGRONOMY ; GENETICS & HEREDITY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000291600800004
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101953]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.NW A&F Univ, Coll Life Sci, Yang Ling 712100, Peoples R China 3.Wuhan Univ, Coll Life Sci, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xinmei,Wu, Jian,Zhang, Hui,et al. Fine mapping of a male sterility gene MS-cd1 in Brassica oleracea[J]. THEORETICAL AND APPLIED GENETICS,2011,123(2):231-238.
APA	Zhang, Xinmei,Wu, Jian,Zhang, Hui,Ma, Yuan,Guo, Aiguang,&Wang, Xiaowu.(2011).Fine mapping of a male sterility gene MS-cd1 in Brassica oleracea.THEORETICAL AND APPLIED GENETICS,123(2),231-238.
MLA	Zhang, Xinmei,et al."Fine mapping of a male sterility gene MS-cd1 in Brassica oleracea".THEORETICAL AND APPLIED GENETICS 123.2(2011):231-238.