A Novel Pepper (Capsicum annuum L.) WRKY Gene, CaWRKY30, Is Involved in Pathogen Stress Responses

doi:10.1007/s12374-011-9171-x

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	A Novel Pepper (Capsicum annuum L.) WRKY Gene, CaWRKY30, Is Involved in Pathogen Stress Responses
	Zheng Jingyuan 1,2; Zou Xuexiao 2; Mao Zhenchuan 1; Xie Bingyan 1
刊名	JOURNAL OF PLANT BIOLOGY
	2011
卷号	54 期号:5 页码:329-337
关键词	WRKY transcription factor Pepper Root-knot nematode Expression analysis
ISSN号	1226-9239
DOI	10.1007/s12374-011-9171-x
通讯作者	Zheng Jingyuan
英文摘要	WRKY proteins are a superfamily of transcription factors involved in many plant processes including plant defense responses to biotic and abiotic stresses. We isolated a WRKY gene from pepper during the incompatible interaction between the pepper cultivar HDA149 and Meloidogyne incognita. The full-length gene, named as CaWRKY30, has a 1,533-bp cDNA sequence and contains an open reading frame of 1,095 bp, encodes a putative polypeptide of 364 amino acids with a theoretical protein size of 41.2 kDa, and contains one WRKY domain followed by a zinc-finger motif. The genomic sequence of CaWRKY30 contains three exons and two introns. Southern blot analysis confirmed that CaWRKY30 exists as a single copy in the pepper cultivar HDA149 genome. Quantitative RT-PCR showed that CaWRKY30 is up-regulated by application of various pathogens including avirulent M. incognita, Tobacco mosaic virus, Ralstonia solanacerum, and Phytophthora capsici Leonian. Furthermore, the transcripts of CaWRKY30 were rapidly induced after treatment with phytohormones salicylic acid (SA). However, the expression of CaWRKY30 was down-regulated by virulent M. incognita and phytohormones methyl jasmonic acid (MeJA). In addition, the nuclear localization of CaWRKY30 was determined when a CaMV35s::CaWRKY30-eGFP fusion construct was expressed in onion epidermal cells. These results suggested that CaWRKY30 might be involved in plant defense mechanisms against the diverse pathogen infection.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000294116300007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101947]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Cent S Univ, Grad Sch, Branch Longping, Changsha 410125, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Zheng Jingyuan,Zou Xuexiao,Mao Zhenchuan,et al. A Novel Pepper (Capsicum annuum L.) WRKY Gene, CaWRKY30, Is Involved in Pathogen Stress Responses[J]. JOURNAL OF PLANT BIOLOGY,2011,54(5):329-337.
APA	Zheng Jingyuan,Zou Xuexiao,Mao Zhenchuan,&Xie Bingyan.(2011).A Novel Pepper (Capsicum annuum L.) WRKY Gene, CaWRKY30, Is Involved in Pathogen Stress Responses.JOURNAL OF PLANT BIOLOGY,54(5),329-337.
MLA	Zheng Jingyuan,et al."A Novel Pepper (Capsicum annuum L.) WRKY Gene, CaWRKY30, Is Involved in Pathogen Stress Responses".JOURNAL OF PLANT BIOLOGY 54.5(2011):329-337.