Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita

doi:10.1007/s11274-014-1781-2

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita
	Yao, Yu-Rong 1; Tian, Xue-Liang 1,2; Shen, Bao-Ming 1,3; Mao, Zhen-Chuan 1; Chen, Guo-hua 1; Xie, Bing-Yan 1
刊名	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
	2015
卷号	31 期号:4 页码:549-556
关键词	Acremonium implicatum Biocontrol Endophytic Fungus gfp Parasitic Root-knot nematode
ISSN号	0959-3993
DOI	10.1007/s11274-014-1781-2
通讯作者	Yao, Yu-Rong
英文摘要	Acremonium implicatum is an endophytic fungus with biocontrol potential against Meloidogyne incognita based on its opportunistic egg-parasitic, hatching inhibition, and toxic properties. To understand its mode of plant endophytism and opportunistic egg parasitism, GFP-tagged A. implicatum was constructed by PEG-mediated protoplast transformation. By laser scanning confocal microscopy (LSCM), we evaluated the endophytism and opportunistic egg parasitism of a stable gfp transformant (Acr-1). Acr-1 could colonize epidermal tissue, cortical tissue, and xylem of roots and form a mutualistic symbiosis with tomato host plants. LSCM of Acr-1 infecting M. incognita eggs revealed that hyphae penetrated the shell and grew inside eggs to form trophic hyphae. A large number of hyphae enveloped parasitized eggs. In addition, the egg shell integrity was destroyed by fungal penetration. The percentage of egg parasitism was 33.8 %. There were no marked differences between the wild type and mutant in nematode second-stage juvenile mortality and egg hatching and in fungal control efficiency in a pot experiment. In conclusion, gfp-transformation did not change the nematicidal activity of A. implicatum and is a tool to examine the mode of plant endophytism and opportunistic egg parasitism of A. implicatum.
学科主题	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000351294200002
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101798]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Henan Inst Sci & Technol, Coll Resources & Environm, Xinxiang 453003, Peoples R China 3.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Yao, Yu-Rong,Tian, Xue-Liang,Shen, Bao-Ming,et al. Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2015,31(4):549-556.
APA	Yao, Yu-Rong,Tian, Xue-Liang,Shen, Bao-Ming,Mao, Zhen-Chuan,Chen, Guo-hua,&Xie, Bing-Yan.(2015).Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,31(4),549-556.
MLA	Yao, Yu-Rong,et al."Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 31.4(2015):549-556.