Expression patterns, molecular markers and genetic diversity of insect-susceptible and resistant Barbarea genotypes by comparative transcriptome analysis

doi:10.1186/s12864-015-1609-y

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Expression patterns, molecular markers and genetic diversity of insect-susceptible and resistant Barbarea genotypes by comparative transcriptome analysis
	Zhang, Xiaohui 1,2; Liu, Tongjin 1,2; Wei, Xiaochun 1,2,3; Qiu, Yang 1,2; Song, Jiangping 1,2; Wang, Haiping 1,2; Shen, Di 1,2; Agerbirk, Niels 4; Li, Xixiang 1,2
刊名	BMC GENOMICS
	2015
卷号	16 页码:-
关键词	Barbarea vulgaris Transcriptome Diamondback moth Expression pattern Molecular marker Genetic diversity Saponin biosynthesis
ISSN号	1471-2164
DOI	10.1186/s12864-015-1609-y
通讯作者	Zhang, Xiaohui
英文摘要	Background: Barbarea vulgaris contains two genotypes: the glabrous type (G-type), which confers resistance to the diamondback moth (DBM) and other insect pests, and the pubescent type (P-type), which is susceptible to the DBM. Herein, the transcriptomes of P-type B. vulgaris before and after DBM infestation were subjected to Illumina (Solexa) pyrosequencing and comparative analysis.Results: 5.0 gigabase pairs of clean nucleotides were generated. Non-redundant unigenes (33,721) were assembled and 94.1 % of them were annotated. Compared with our previous G-type transcriptome, the expression patterns of many insect responsive genes, including those related to secondary metabolism, phytohormones and transcription factors, which were significantly induced by DBM in G-type plants, were less sensitive to DBM infestation in P-type plants. The genes of the triterpenoid saponin pathway were identified in both G- and P-type plants. The upstream genes of the pathway showed similar expression patterns between the two genotypes. However, gene expression for two downstream enzymes, the glucosyl transferase (UGT73C11) and an oxidosqualene cyclase (OSC), were significantly upregulated in the P-type compared with the G-type plant. The homologous genes from P- and G-type plants were detected by BLAST unigenes with a cutoff level E-value < e(-10). 12,980 gene families containing 26,793 P-type and 36,944 G-type unigenes were shared by the two types of B. vulgaris. 38,397 single nucleotide polymorphisms (SNPs) were found in 9,452 orthologous genes between the P- and G-type plants. We also detected 5,105 simple sequence repeats (SSRs) in the B. vulgaris transcriptome, comprising mono-nucleotide-repeats (2,477; 48.5 %) and triple-nucleotide-repeats (1,590; 31.1 %). Of these, 1,657 SSRs displayed polymorphisms between the P- and G-type. Consequently, 913 SSR primer pairs were designed with a resolution of more than two nucleotides. We randomly chose 30 SSRs to detect the genetic diversity of 32 Barbarea germplasms. The distance tree showed that these accessions were clearly divided into groups, with the G-type grouping with available Western and Central European B. vulgaris accessions in contrast to the P-type accession, B. stricta and B. verna.Conclusions: These data represent useful information for pest-resistance gene mining and for the investigation of the molecular basis of plant-pest interactions.
学科主题	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000357071700001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101786]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops, Beijing 100081, Peoples R China 3.Henan Acad Agr Sci, Inst Hort, Zhengzhou 450002, Peoples R China 4.Univ Copenhagen, Dept Plant & Environm Sci, DK-1871 Frederiksberg C, Denmark
推荐引用方式 GB/T 7714	Zhang, Xiaohui,Liu, Tongjin,Wei, Xiaochun,et al. Expression patterns, molecular markers and genetic diversity of insect-susceptible and resistant Barbarea genotypes by comparative transcriptome analysis[J]. BMC GENOMICS,2015,16:-.
APA	Zhang, Xiaohui.,Liu, Tongjin.,Wei, Xiaochun.,Qiu, Yang.,Song, Jiangping.,...&Li, Xixiang.(2015).Expression patterns, molecular markers and genetic diversity of insect-susceptible and resistant Barbarea genotypes by comparative transcriptome analysis.BMC GENOMICS,16,-.
MLA	Zhang, Xiaohui,et al."Expression patterns, molecular markers and genetic diversity of insect-susceptible and resistant Barbarea genotypes by comparative transcriptome analysis".BMC GENOMICS 16(2015):-.