Comparative Proteomics Analyses of Two Races of Fusarium oxysporum f. sp conglutinans that Differ in Pathogenicity

doi:10.1038/srep13663

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Comparative Proteomics Analyses of Two Races of Fusarium oxysporum f. sp conglutinans that Differ in Pathogenicity
	Li, Erfeng 1; Ling, Jian 1; Wang, Gang 1; Xiao, Jiling 1; Yang, Yuhong 1; Mao, Zhenchuan 1; Wang, Xuchu 2; Xie, Bingyan 1
刊名	SCIENTIFIC REPORTS
	2015
卷号	5 页码:-
ISSN号	2045-2322
DOI	10.1038/srep13663
通讯作者	Li, Erfeng
英文摘要	Fusarium oxysporum is a soil-inhabiting fungus that induces vascular wilt and root rot in a variety of plants. F. oxysporum f. sp. conglutinans (Foc), which comprises two races, can cause wilt disease in cabbage. Compared with race 1 (52557(-TM), R1), race 2 (58385(-TM), R2) exhibits much stronger pathogenicity. Here, we provide the first proteome reference maps for Foc mycelium and conidia and identify 145 proteins with different abundances among the two races. Of these proteins, most of the high-abundance proteins in the R2 mycelium and conidia are involved in carbohydrate, amino acid and ion metabolism, which indicates that these proteins may play important roles in isolate R2's stronger pathogenicity. The expression levels of 20 typical genes demonstrate similarly altered patterns compared to the proteomic analysis. The protein glucanosyltransferase, which is involved in carbohydrate metabolism, was selected for research. We knocked out the corresponding gene (gas1) and found that Foc-Delta gas1 significantly reduced growth rate and virulence compared with wild type isolates. These results deepened our understanding of the proteins related to F. oxysporum pathogenicity in cabbage Fusarium wilt and provided new opportunities to control this disease.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000360534500001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101778]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
推荐引用方式 GB/T 7714	Li, Erfeng,Ling, Jian,Wang, Gang,et al. Comparative Proteomics Analyses of Two Races of Fusarium oxysporum f. sp conglutinans that Differ in Pathogenicity[J]. SCIENTIFIC REPORTS,2015,5:-.
APA	Li, Erfeng.,Ling, Jian.,Wang, Gang.,Xiao, Jiling.,Yang, Yuhong.,...&Xie, Bingyan.(2015).Comparative Proteomics Analyses of Two Races of Fusarium oxysporum f. sp conglutinans that Differ in Pathogenicity.SCIENTIFIC REPORTS,5,-.
MLA	Li, Erfeng,et al."Comparative Proteomics Analyses of Two Races of Fusarium oxysporum f. sp conglutinans that Differ in Pathogenicity".SCIENTIFIC REPORTS 5(2015):-.