MicroRNA and target gene responses to salt stress in grafted cucumber seedlings

doi:10.1007/s11738-016-2070-5

CORC > 中国农业科学院 > 蔬菜花卉研究所

	MicroRNA and target gene responses to salt stress in grafted cucumber seedlings
	Li, Yansu 1; Li, Chaohan 1,2; Bai, Longqiang 1; He, Chaoxing 1; Yu, Xianchang 1
刊名	ACTA PHYSIOLOGIAE PLANTARUM
	2016
卷号	38 期号:2 页码:-
关键词	Cucumber Graft Salt stress miRNA Target genes of miRNA Expression of miRNA and the targets
ISSN号	0137-5881
DOI	10.1007/s11738-016-2070-5
通讯作者	Li, Yansu
英文摘要	Grafting is widely applied to enhance cucumber growth and tolerance to various biotic and abiotic adversities. Except for regulating plant growth and plant development, microRNAs (miRNAs) respond to various adversities through negative mediation of target gene expression at the post-transcriptional level. In the present study, cucumber (Cucumis sativus) scions were grafted onto pumpkin (Cucurbita moschata) rootstocks to characterize the molecular mechanisms of miRNAs and target regulatory networks that confer increased salt stress tolerance. The expression levels of 17 miRNAs and their predicted target genes were determined by quantitative real-time PCR using auto-grafted (self-grafted cucumber seedlings) and hetero-grafted (cucumber scion grafted onto pumpkin rootstock) cucumber seedlings after 24 h of 100 mmol l(-1) NaCl treatment. Compared with that of auto-grafted cucumber seedlings, the expression level of most miRNAs in the leaves of hetero-grafted cucumber seedlings increased under normal conditions, decreased considerably after 2 h of salt stress, and increased after 6 and 24 h of salt stress. The expression level of most miRNAs in the roots of hetero-grafted cucumber seedlings increased under normal conditions and salt-stressed conditions. Compared with that of auto-grafted cucumber seedlings, the expression level of most target genes in the leaves of hetero-grafted cucumber seedlings decreased under normal and salt-stressed conditions. However, the expression level of most target genes in the roots of hetero-grafted cucumber seedlings increased significantly. These results provide useful information relevant to the functional analysis of miRNAs mediating graft-dependent salt tolerance.
学科主题	Plant Sciences
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000369692100011
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101757]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, 12 Zhongguancun South St, Beijing 100081, Peoples R China 2.Shanghai Acad Agr Sci, Inst Hort Res, 2901 Beidi Rd, Shanghai 201106, Peoples R China
推荐引用方式 GB/T 7714	Li, Yansu,Li, Chaohan,Bai, Longqiang,et al. MicroRNA and target gene responses to salt stress in grafted cucumber seedlings[J]. ACTA PHYSIOLOGIAE PLANTARUM,2016,38(2):-.
APA	Li, Yansu,Li, Chaohan,Bai, Longqiang,He, Chaoxing,&Yu, Xianchang.(2016).MicroRNA and target gene responses to salt stress in grafted cucumber seedlings.ACTA PHYSIOLOGIAE PLANTARUM,38(2),-.
MLA	Li, Yansu,et al."MicroRNA and target gene responses to salt stress in grafted cucumber seedlings".ACTA PHYSIOLOGIAE PLANTARUM 38.2(2016):-.