Characterization of the Fatty Acid Desaturase Genes in Cucumber: Structure, Phylogeny, and Expression Patterns

doi:10.1371/journal.pone.0149917

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Characterization of the Fatty Acid Desaturase Genes in Cucumber: Structure, Phylogeny, and Expression Patterns
	Dong, Chun-Juan; Cao, Ning; Zhang, Zhi-Gang; Shang, Qing-Mao
刊名	PLOS ONE
	2016
卷号	11 期号:3 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0149917
通讯作者	Dong, Chun-Juan
英文摘要	Fatty acid desaturases (FADs) introduce double bonds into the hydrocarbon chains of fatty acids to produce unsaturated fatty acids, and therefore play a critical role in plant development and acclimation to environmental stresses. In this study, 23 full-length FAD genes in cucumber (Cucumis sativus L.) were identified through database searches, including three CsFAB2 genes, two CsFAD2 genes, fourteen CsFAD5 genes, and one gene each for CsFAD3, CsFAD4, CsFAD6 and CsFAD7. These cucumber FAD genes were distributed on all seven chromosomes and two additional scaffolds. Based on a phylogenetic analysis, the cucumber FAD proteins were clustered into five subfamilies with their counterparts from other plants. Gene structures and protein sequences were considerably conserved in each subfamily. All three CsFAB2 proteins shared conserved structure with the known plant soluble FAD proteins. The other cucumber FADs belonged to the membrane-bound FADs and contained three highly conserved histidine boxes. Additionally, the putative endoplasmic reticulum retention signal was found at the C-termini of the CsFAD2 and CsFAD3 proteins, while the N-termini of CsFAD4, CsFAD5, CsFAD6, CsFAD7 and three CsFAB2s contained a predicted chloroplast signal peptide, which was consistent with their associated metabolic pathways. Furthermore, a gene expression analysis showed that CsFAD2 and CsFAD3 were universally expressed in all tested tissues, whereas the other cucumber FAD genes were preferentially expressed in the cotyledons or leaves. The tissue-specific expression patterns of cucumber FAD genes were correlated well with the differences in the fatty acid compositions ofroots and leaves. Finally, the cucumber FAD genes showed a cold-induced and heat-repressed expression pattern, although with distinct regulatory time courses among the different CsFAD members, which indicates the potential roles of the FADs in temperature stress resistance in cucumber.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000371735200043
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101751]
专题	蔬菜花卉研究所
作者单位	Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Hort Crop Biol & Germplasm Innovat, Minist Agr, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Dong, Chun-Juan,Cao, Ning,Zhang, Zhi-Gang,et al. Characterization of the Fatty Acid Desaturase Genes in Cucumber: Structure, Phylogeny, and Expression Patterns[J]. PLOS ONE,2016,11(3):-.
APA	Dong, Chun-Juan,Cao, Ning,Zhang, Zhi-Gang,&Shang, Qing-Mao.(2016).Characterization of the Fatty Acid Desaturase Genes in Cucumber: Structure, Phylogeny, and Expression Patterns.PLOS ONE,11(3),-.
MLA	Dong, Chun-Juan,et al."Characterization of the Fatty Acid Desaturase Genes in Cucumber: Structure, Phylogeny, and Expression Patterns".PLOS ONE 11.3(2016):-.