Development of a novel allele-specific Rfo marker and creation of Ogura CMS fertility-restored interspecific hybrids in Brassica oleracea

doi:10.1007/s00122-016-2728-9

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Development of a novel allele-specific Rfo marker and creation of Ogura CMS fertility-restored interspecific hybrids in Brassica oleracea
	Yu, Hai-long; Fang, Zhi-yuan; Liu, Yu-mei; Yang, Li-mei; Zhuang, Mu; Lv, Hong-hao; Li, Zhan-sheng; Han, Feng-qing; Liu, Xiao-ping; Zhang, Yang-yong
刊名	THEORETICAL AND APPLIED GENETICS
	2016
卷号	129 期号:8 页码:1625-1637
ISSN号	0040-5752
DOI	10.1007/s00122-016-2728-9
通讯作者	Yu, Hai-long
英文摘要	A novel allele-specific Rfo marker was developed and proved to be effective for MAS of Rfo gene in B. oleracea background and six Ogu-CMS fertility-restored interspecific hybrids were created for the first time.Ogura cytoplasmic male sterility (Ogu-CMS) has been extensively used for Brassica oleracea hybrid production. However, because of maternal inheritance, all the hybrids produced by CMS lines are male sterile and cannot be self-pollinated, which prohibits germplasm maintenance and innovation. This problem can be overcome by using the Ogu-CMS restorer line, but restorer material is absent in B. oleracea crops. Here, Rfo, a fertility-restored gene of Ogu-CMS, was transferred from rapeseed restorer lines into a Chinese kale Ogu-CMS line using interspecific hybridization combined with embryo rescue. Nine interspecific, triploid plant progenies were identified at morphological and ploidy level, with phenotypes intermediate between those of rapeseed and Chinese kale. Because the Rfo marker (Hu et al., Mol Breeding 22:663-674, 2008) cannot distinguish the Rfo and its homologies under a B. oleracea background, a novel allele-specific Rfo marker was developed based on the BLAST analysis of highly homologous Rfo sequences in B. oleracea. Screening using the novel Rfo marker found that six interspecific hybrids carrying Rfo were also fertile, although fertility varied during different flowering periods. Furthermore, BC1 offsprings with the Rfo gene were selected with the allele-specific Rfo marker and showed restored fertility. These results indicated that the novel allele-specific marker could be used for the MAS of Rfo gene in B. oleracea, and this study lays the foundation for the development of Ogu-CMS restorer material in cabbage and its related other subspecies.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000379857800013
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101729]
专题	蔬菜花卉研究所
作者单位	Chinese Acad Agr Sci, Inst Vegetables & Flowers, 12 ZhongGuanCun South St, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Yu, Hai-long,Fang, Zhi-yuan,Liu, Yu-mei,et al. Development of a novel allele-specific Rfo marker and creation of Ogura CMS fertility-restored interspecific hybrids in Brassica oleracea[J]. THEORETICAL AND APPLIED GENETICS,2016,129(8):1625-1637.
APA	Yu, Hai-long.,Fang, Zhi-yuan.,Liu, Yu-mei.,Yang, Li-mei.,Zhuang, Mu.,...&Zhang, Yang-yong.(2016).Development of a novel allele-specific Rfo marker and creation of Ogura CMS fertility-restored interspecific hybrids in Brassica oleracea.THEORETICAL AND APPLIED GENETICS,129(8),1625-1637.
MLA	Yu, Hai-long,et al."Development of a novel allele-specific Rfo marker and creation of Ogura CMS fertility-restored interspecific hybrids in Brassica oleracea".THEORETICAL AND APPLIED GENETICS 129.8(2016):1625-1637.