Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance

doi:10.1007/s12010-012-9758-9

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	Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance
	Li, Zuming 1,2; Bai, Zhihui 1; Zhang, Baoguo 1; Li, Baojv 3; Jin, Bo 4; Zhang, Michael 5; Lin, Francis 5; Zhang, Hongxun 1
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2012
卷号	167 期号:8 页码:2241-2256
关键词	Bacillus clausii Alkaline pectin lyase Sugar beet pulp Mass spectrometric sequencing Plant disease resistance
ISSN号	0273-2289
DOI	10.1007/s12010-012-9758-9
通讯作者	Li, Zuming
英文摘要	Alkaline pectin lyase (PNL) shows potential as a biological control agent against several plant diseases. We isolated and characterized a new Bacillus clausii strain that can produce 4,180 U/g of PNL using sugar beet pulp as a carbon source and inducer. The PNL was purified to apparent homogeneity using ultrafiltration, ammonium sulfate fractionation, DEAE Sepharose Fast Flow, and Sephadex G-75 gel filtration. The purified PNL was found to be a monomeric protein with a molecular weight of 35 kDa, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It demonstrated optimal activity with K (m) of 0.87 mg/ml at pH 10.0 and 60 A degrees C. The enzyme is stable in the pH range of 8.0-10.0 and temperature a parts per thousand currency sign40 A degrees C. Ca2+ was found to stimulate the enzymatic activity of the PNL by up to 410 %. Mass spectrometric results gave 38 % match coverage with pectate lyase from B. clausii KSM-K16 (gi\|56961845). The PNL was found to elicit disease resistance in cucumber seedlings, suggesting that it may have applications in biocontrol and sustainable agriculture.
学科主题	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY
语种	英语
出版者	HUMANA PRESS INC
WOS记录号	WOS:000307537500009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101093]
专题	蔬菜花卉研究所
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China 2.Beijing Union Univ, Coll Arts & Sci, Beijing 100191, Peoples R China 3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 4.Univ Adelaide, Sch Chem Engn, Adelaide, SA 5005, Australia 5.Univ Manitoba, Dept Phys & Astron, Winnipeg, MB R3T 2N2, Canada
推荐引用方式 GB/T 7714	Li, Zuming,Bai, Zhihui,Zhang, Baoguo,et al. Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012,167(8):2241-2256.
APA	Li, Zuming.,Bai, Zhihui.,Zhang, Baoguo.,Li, Baojv.,Jin, Bo.,...&Zhang, Hongxun.(2012).Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,167(8),2241-2256.
MLA	Li, Zuming,et al."Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 167.8(2012):2241-2256.