Characterization and expression of daf-9 and daf-12 genes in the pinewood nematode, Bursaphelenchus xylophilus

doi:10.1111/efp.12011

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Characterization and expression of daf-9 and daf-12 genes in the pinewood nematode, Bursaphelenchus xylophilus
	Wang, D. -D.1,2; Cheng, X. -Y.3; Wang, Y. -S.2; Pan, H. -Y.1; Xie, B. -Y.2
刊名	FOREST PATHOLOGY
	2013
卷号	43 期号:2 页码:144-152
ISSN号	1437-4781
DOI	10.1111/efp.12011
通讯作者	Wang, D. -D.
英文摘要	Dauer formation is a mechanism by which nematodes cope with difficult environmental circumstances. DAF-encoding genes are known to control the formation of dauer larvae in several nematode species. In this article, two important daf genes were isolated from the invasive plant parasitic nematode, Bursaphelenchus xylophilus, which has two dauer larval stages for overwintering and transmitting by vector beetles in its life cycle. A 1915-bp full-length cDNA of Bx-daf-9 was obtained, containing a 1533-bp open reading frame (ORF), encoding a polypeptide of 510 amino acids. A 3966-bp full-length cDNA of Bx-daf-12 was obtained, containing a 2712-bp ORF, which encoded a polypeptide of 903 amino acids. The structures of both genes in nematodes were compared. A 2758-bp promoter region of Bx-daf-9 and a 2459-bp promoter region of Bx-daf-12 were obtained, with eight putative response elements binding transcription factors in each promoter region. The quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) was performed to detect the mRNA expression levels of the two genes in different stages of the nematode. The result showed that expression levels of the two genes were low in egg stage and up-regulated five to seven times in larval and adult stages. However, Bx-daf-9 expression in the third dispersal stage was relatively low. Expression of daf-12 in the fourth dispersal stage was extraordinarily high. The results imply that daf-9 and daf-12 perhaps have special roles in the dispersal stages of B.xylophilus.
学科主题	Forestry ; FORESTRY
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000317302300006
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101061]
专题	蔬菜花卉研究所
作者单位	1.Jilin Univ, Coll Plant Sci, Changchun 130062, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 3.Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China
推荐引用方式 GB/T 7714	Wang, D. -D.,Cheng, X. -Y.,Wang, Y. -S.,et al. Characterization and expression of daf-9 and daf-12 genes in the pinewood nematode, Bursaphelenchus xylophilus[J]. FOREST PATHOLOGY,2013,43(2):144-152.
APA	Wang, D. -D.,Cheng, X. -Y.,Wang, Y. -S.,Pan, H. -Y.,&Xie, B. -Y..(2013).Characterization and expression of daf-9 and daf-12 genes in the pinewood nematode, Bursaphelenchus xylophilus.FOREST PATHOLOGY,43(2),144-152.
MLA	Wang, D. -D.,et al."Characterization and expression of daf-9 and daf-12 genes in the pinewood nematode, Bursaphelenchus xylophilus".FOREST PATHOLOGY 43.2(2013):144-152.