Identification of sequence-related amplified polymorphism and insertion-deletion markers linked to the male fertility restorer gene of pol-like CMS06J45 in heading Chinese cabbage (Brassica rapa subsp pekinensis)

doi:10.4238/2014.November.14.4

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Identification of sequence-related amplified polymorphism and insertion-deletion markers linked to the male fertility restorer gene of pol-like CMS06J45 in heading Chinese cabbage (Brassica rapa subsp pekinensis)
	Xu, X. Y.1,2; Zhang, Y.1; Zhang, L. G.1; Fang, Z. Y.3
刊名	GENETICS AND MOLECULAR RESEARCH
	2014
卷号	13 期号:4 页码:9606-9614
关键词	BrRfp Heading Chinese cabbage Insertion-deletion Sequence-related amplified polymorphism CMS06J4
ISSN号	1676-5680
DOI	10.4238/2014.November.14.4
通讯作者	Xu, X. Y.
英文摘要	In order to map the restorer gene BrRfp of the polima (pol)-like cytoplasmic male sterility (CMS) 06J45 line in heading Chinese cabbage, an F-2 segregating population with 258 individuals of CMS06J45 and the restorer line 01S325 were tested by sequence-related amplified polymorphism (SRAP) and insertion-deletion (InDel) technologies combined with the bulked segregant analysis method. As a result, two SRAP markers, me3em3.366 and pm88bg5.263, that were linked with the BrRfp gene were identified from 463 SRAP primer pairs. By cloning, sequencing, and basic local alignment search tool analysis, the two markers were targeted to the BGIScaffold000053 of Brassica rapa in the Brassica database. Using the BGIScaffold000053 sequence, four InDel primer pairs were designed and identified to be linked with the BrRfp gene in this population. Linkage analysis showed that these markers were distributed on both sides of the BrRfp gene, the linkage distances of two nearest markers InDel878.1125 and InDel920.713 were 0.82 and 0.46 cM, respectively, and the BrRfp gene was restricted to a 243-kb genomic region of B. rapa. These specific markers provided basic information for map-based cloning of the BrRfp gene and will be very valuable for the marker-assisted selection of a new restorer line in heading Chinese cabbage.
学科主题	Biochemistry & Molecular Biology ; Genetics & Heredity
语种	英语
出版者	FUNPEC-EDITORA
WOS记录号	WOS:000345119600080
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101005]
专题	蔬菜花卉研究所
作者单位	1.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China 2.Shanxi Agr Univ, Coll Hort, Taigu, Shanxi, Peoples R China 3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Xu, X. Y.,Zhang, Y.,Zhang, L. G.,et al. Identification of sequence-related amplified polymorphism and insertion-deletion markers linked to the male fertility restorer gene of pol-like CMS06J45 in heading Chinese cabbage (Brassica rapa subsp pekinensis)[J]. GENETICS AND MOLECULAR RESEARCH,2014,13(4):9606-9614.
APA	Xu, X. Y.,Zhang, Y.,Zhang, L. G.,&Fang, Z. Y..(2014).Identification of sequence-related amplified polymorphism and insertion-deletion markers linked to the male fertility restorer gene of pol-like CMS06J45 in heading Chinese cabbage (Brassica rapa subsp pekinensis).GENETICS AND MOLECULAR RESEARCH,13(4),9606-9614.
MLA	Xu, X. Y.,et al."Identification of sequence-related amplified polymorphism and insertion-deletion markers linked to the male fertility restorer gene of pol-like CMS06J45 in heading Chinese cabbage (Brassica rapa subsp pekinensis)".GENETICS AND MOLECULAR RESEARCH 13.4(2014):9606-9614.