Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium

	Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium
	Wang BQ ; Li B ; Wang ZX ; Xu GB ; Wang Q ; Dong SJ
刊名	analytical chemistry
	1999
卷号	71 期号:10 页码:1935-1939
关键词	REDUCED NICOTINAMIDE COENZYMES MODIFIED ELECTRODES HORSERADISH-PEROXIDASE ELECTROCATALYTIC OXIDATION ENZYME ELECTRODES METHYLENE GREEN GLUCOSE-OXIDASE GLASS COMPOSITE ENCAPSULATION
ISSN号	0003-2700
通讯作者	dong sj
中文摘要	an acid-stable soybean-peroxidase biosensor was devel oped by immobilizing the enzyme in a sol-gel thin film. methylene blue was used as a mediator because of its high electron-transfer efficiency. the sol-gel thin film and enzyme membrane were characterized by ft-ir, and the effects of ph, operating potential, and temperature were explored for optimum analytical performance by using the amperometric method. the h2o2 sensor exhibited a fast response (5 s), high sensitivity (27.5 mu a/mm), as well as good thermostability and long-term stability. in addition, the performance of the biosensor was investigated using flow-injection analysis (fia).
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000080304700003
公开日期	2010-11-04
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/21879]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang BQ,Li B,Wang ZX,et al. Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium[J]. analytical chemistry,1999,71(10):1935-1939.
APA	Wang BQ,Li B,Wang ZX,Xu GB,Wang Q,&Dong SJ.(1999).Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium.analytical chemistry,71(10),1935-1939.
MLA	Wang BQ,et al."Sol-gel thin-film immobilized soybean peroxidase biosensor for the amperometric determination of hydrogen peroxide in acid medium".analytical chemistry 71.10(1999):1935-1939.