Sol-gel-derived amperometric biosensor for hydrogen peroxide based on methylene green incorporated in Nafion film

	Sol-gel-derived amperometric biosensor for hydrogen peroxide based on methylene green incorporated in Nafion film
	Wang BQ ; Dong SJ
刊名	talanta
	2000
卷号	51 期号:3 页码:565-572
关键词	REGENERATED SILK FIBROIN HORSERADISH-PEROXIDASE ENZYME ELECTRODE IMMOBILIZATION MATRIX GRAPHITE ELECTRODE GLASS GLUCOSE SENSOR FABRICATION ENCAPSULATION
ISSN号	0039-9140
通讯作者	dong sj
中文摘要	a hydrogen peroxide biosensor was fabricated by coating a sol-gel-peroxidase layer onto a nafion-methylene green modified electrode. immobilization of methylene green (mg) was attributed to the electrostatic force between mg(+) and the negatively charged sulfonic acid groups in nafion polymer, whereas immobilization of horseradish peroxidase was attributed to the encapsulation function of the silica sol-gel network. cyclic voltammetry and chronoamperometry were employed to demonstrate the feasibility of electron transfer between sol-gel-immobilized peroxidase and a glassy carbon electrode. performance of the sensor was evaluated with respect to response time, sensitivity as well as operational stability. the enzyme electrode has a sensitivity of 13.5 mu a mm(-1) with a detection limit of 1.0 x 10(-7) m h2o2, and the sensor achieved 95% of the steady-state current within 20 s. (c) 2000 elsevier science b.v. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000085712300016
公开日期	2010-11-04
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/19861]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang BQ,Dong SJ. Sol-gel-derived amperometric biosensor for hydrogen peroxide based on methylene green incorporated in Nafion film[J]. talanta,2000,51(3):565-572.
APA	Wang BQ,&Dong SJ.(2000).Sol-gel-derived amperometric biosensor for hydrogen peroxide based on methylene green incorporated in Nafion film.talanta,51(3),565-572.
MLA	Wang BQ,et al."Sol-gel-derived amperometric biosensor for hydrogen peroxide based on methylene green incorporated in Nafion film".talanta 51.3(2000):565-572.